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miRNA: 3'- -CUGUGGUUGCACAgCGAcgUCCGGGu -5'
miRNA: 3'- cUGUGGUugCA-CA---------GCGACGUCCGGGu -5'
miRNA: 3'- cugUGGuUGCaCAGCGACGUCCGGGU- -5'
miRNA: 3'- -CUGUGGUUGCACAGCGACGUCCGGGU- -5'
Results 141 - 144 of 144 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 21385 | 5' | -57.6 | NC_004812.1 | + | 128915 | 0.77 | 0.27958 |
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Target: 5'- gGGCGCCGGCGUGUgGCU--GGGCCCc -3' miRNA: 3'- -CUGUGGUUGCACAgCGAcgUCCGGGu -5' |
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| 21385 | 5' | -57.6 | NC_004812.1 | + | 86480 | 0.77 | 0.273089 |
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Target: 5'- uGCGCCAugGUcGUugacggagcCGCUGCGGGCCCc -3' miRNA: 3'- cUGUGGUugCA-CA---------GCGACGUCCGGGu -5' |
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| 21385 | 5' | -57.6 | NC_004812.1 | + | 83069 | 0.81 | 0.159101 |
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Target: 5'- --uGCCcGCG-GUCGCUGCGGGCCCGc -3' miRNA: 3'- cugUGGuUGCaCAGCGACGUCCGGGU- -5' |
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| 21385 | 5' | -57.6 | NC_004812.1 | + | 135478 | 1.09 | 0.001992 |
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Target: 5'- cGACACCAACGUGUCGCUGCAGGCCCAg -3' miRNA: 3'- -CUGUGGUUGCACAGCGACGUCCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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