miRNA: 3'- cUGUGGU-UGCACaGCGA-----CGUCCGG-GU- -5'
miRNA: 3'- -CUGUGGUUGCA-CaGCG--ACGu-CCGGGu -5'
miRNA: 3'- -CUGUGGU------------UGCacaGCGaCGUCC-GGGU- -5'
miRNA: 3'- cUGUGGUUGCa-----C-AGCGaCGUCCGgGU- -5'
Results 141 - 144 of 144 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21385 | 5' | -57.6 | NC_004812.1 | + | 154381 | 0.68 | 0.720909 |
|
Target: 5'- cGCGCCGcGCGUG-CGCUgggccGCGGGCCgCGg -3' miRNA: 3'- cUGUGGU-UGCACaGCGA-----CGUCCGG-GU- -5' |
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| 21385 | 5' | -57.6 | NC_004812.1 | + | 155151 | 0.69 | 0.640228 |
|
Target: 5'- gGACGCCGGCGUcGcCGCcgUGCccGGCCCu -3' miRNA: 3'- -CUGUGGUUGCA-CaGCG--ACGu-CCGGGu -5' |
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| 21385 | 5' | -57.6 | NC_004812.1 | + | 155274 | 0.66 | 0.822971 |
|
Target: 5'- gGACGCCAaaccggggagggGCGgggCGCgGCGGGgCCCGg -3' miRNA: 3'- -CUGUGGU------------UGCacaGCGaCGUCC-GGGU- -5' |
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| 21385 | 5' | -57.6 | NC_004812.1 | + | 156158 | 0.67 | 0.769161 |
|
Target: 5'- cGCGCCGGCGgcccggGcUCGCgGCGGGCgCGc -3' miRNA: 3'- cUGUGGUUGCa-----C-AGCGaCGUCCGgGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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