miRNA: 3'- -CUGUGGUUGCacaGCGa------CGUCCGGgu -5'
miRNA: 3'- cUGUGGU---UGCACAGcGACG-----------UCCGGgu -5'
miRNA: 3'- cuGUGGUUGCaCAGCGAcgUCCG-GGU- -5'
miRNA: 3'- cUGUGGuUGCaca--GCGa--CGUCCGGGU- -5'
Results 141 - 144 of 144 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21385 | 5' | -57.6 | NC_004812.1 | + | 129226 | 0.67 | 0.787677 |
|
Target: 5'- cGGCGCCGGCGgagCGCaccuggcGCGGGCCg- -3' miRNA: 3'- -CUGUGGUUGCacaGCGa------CGUCCGGgu -5' |
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| 21385 | 5' | -57.6 | NC_004812.1 | + | 60125 | 0.67 | 0.79673 |
|
Target: 5'- cGCGCCAgcgGCGUGUC-CUGCcccuccaggagGGGCCa- -3' miRNA: 3'- cUGUGGU---UGCACAGcGACG-----------UCCGGgu -5' |
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| 21385 | 5' | -57.6 | NC_004812.1 | + | 127513 | 0.67 | 0.79673 |
|
Target: 5'- -cCGCCGGCG-GUCGCUcgGGGCgCCGa -3' miRNA: 3'- cuGUGGUUGCaCAGCGAcgUCCG-GGU- -5' |
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| 21385 | 5' | -57.6 | NC_004812.1 | + | 18543 | 0.66 | 0.847662 |
|
Target: 5'- cGCGCUcGCGgccagCGCggcGCGGGCCCGc -3' miRNA: 3'- cUGUGGuUGCaca--GCGa--CGUCCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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