Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21400 | 5' | -56.8 | NC_004812.1 | + | 33634 | 0.66 | 0.87541 |
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Target: 5'- aUGGCCCCCGAg---CUCUacgUGUacGUGGa -3' miRNA: 3'- gACCGGGGGCUacuaGAGG---ACG--UACU- -5' |
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| 21400 | 5' | -56.8 | NC_004812.1 | + | 126017 | 0.66 | 0.882507 |
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Target: 5'- -cGGCCCCCGGUccccgcuGUCUCCcGCcccggGAg -3' miRNA: 3'- gaCCGGGGGCUAc------UAGAGGaCGua---CU- -5' |
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| 21400 | 5' | -56.8 | NC_004812.1 | + | 147967 | 0.66 | 0.882507 |
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Target: 5'- --cGCCCCCGcgGcgCUCCgUGCucAUGGc -3' miRNA: 3'- gacCGGGGGCuaCuaGAGG-ACG--UACU- -5' |
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| 21400 | 5' | -56.8 | NC_004812.1 | + | 509 | 0.66 | 0.882507 |
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Target: 5'- -cGGCCCCCGGUccccgcuGUCUCCcGCcccggGAg -3' miRNA: 3'- gaCCGGGGGCUAc------UAGAGGaCGua---CU- -5' |
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| 21400 | 5' | -56.8 | NC_004812.1 | + | 88526 | 0.66 | 0.889383 |
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Target: 5'- cCUGGCCUCCGAgGAgUUCCgggaGC-UGAa -3' miRNA: 3'- -GACCGGGGGCUaCUaGAGGa---CGuACU- -5' |
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| 21400 | 5' | -56.8 | NC_004812.1 | + | 55855 | 0.66 | 0.889383 |
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Target: 5'- aCUGcGCCCUCGGucUGGUCcUCUGCAg-- -3' miRNA: 3'- -GAC-CGGGGGCU--ACUAGaGGACGUacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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