Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2143 | 5' | -64.8 | NC_001362.1 | + | 6088 | 0.69 | 0.029798 |
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Target: 5'- gGGCCCCGGGGGCgAgGAauaggGGGCCu -3' miRNA: 3'- -CUGGGGCCCCUGgUgCUgggg-UCCGG- -5' |
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| 2143 | 5' | -64.8 | NC_001362.1 | + | 3327 | 0.69 | 0.027966 |
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Target: 5'- aACCCUaGGGGACCuCGGauaUCGGGCCu -3' miRNA: 3'- cUGGGG-CCCCUGGuGCUgg-GGUCCGG- -5' |
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| 2143 | 5' | -64.8 | NC_001362.1 | + | 167 | 0.69 | 0.025996 |
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Target: 5'- aGACCCCugcccaGGGACCAcCGACCCacCAccgggagguaagcuGGCCa -3' miRNA: 3'- -CUGGGGc-----CCCUGGU-GCUGGG--GU--------------CCGG- -5' |
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| 2143 | 5' | -64.8 | NC_001362.1 | + | 8026 | 0.7 | 0.025424 |
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Target: 5'- cACCgCGGuucGGCCcCGGCCCgGGGCCa -3' miRNA: 3'- cUGGgGCCc--CUGGuGCUGGGgUCCGG- -5' |
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| 2143 | 5' | -64.8 | NC_001362.1 | + | 371 | 0.72 | 0.015764 |
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Target: 5'- gGACUUCGGGGGCCAuuuuugUGGCCC--GGCCa -3' miRNA: 3'- -CUGGGGCCCCUGGU------GCUGGGguCCGG- -5' |
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| 2143 | 5' | -64.8 | NC_001362.1 | + | 6265 | 0.73 | 0.013013 |
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Target: 5'- -uUCCCGGGGGCgAugUGACCCgGGGCa -3' miRNA: 3'- cuGGGGCCCCUGgU--GCUGGGgUCCGg -5' |
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| 2143 | 5' | -64.8 | NC_001362.1 | + | 8043 | 0.74 | 0.009755 |
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Target: 5'- aACCgCGGuGACCAucuguucuUGGCCCCGGGCCg -3' miRNA: 3'- cUGGgGCCcCUGGU--------GCUGGGGUCCGG- -5' |
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| 2143 | 5' | -64.8 | NC_001362.1 | + | 8089 | 0.81 | 0.0023 |
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Target: 5'- gGGCCauauCUGGGGACCAucuguucuUGGCCCCGGGCCg -3' miRNA: 3'- -CUGG----GGCCCCUGGU--------GCUGGGGUCCGG- -5' |
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| 2143 | 5' | -64.8 | NC_001362.1 | + | 2183 | 1.1 | 6e-06 |
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Target: 5'- gGACCCCGGGGACCACGACCCCAGGCCu -3' miRNA: 3'- -CUGGGGCCCCUGGUGCUGGGGUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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