Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2144 | 5' | -54.7 | NC_001362.1 | + | 6916 | 0.67 | 0.1594 |
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Target: 5'- aGCUGACCCu--GUCCgaagugacuggacggGGACUCUg- -3' miRNA: 3'- aCGACUGGGuuuUAGGa--------------CCUGGGGau -5' |
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| 2144 | 5' | -54.7 | NC_001362.1 | + | 2959 | 0.67 | 0.158885 |
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Target: 5'- uUGUUGACUUcucucAGAUCCUGGACaggCCUAu -3' miRNA: 3'- -ACGACUGGGu----UUUAGGACCUGg--GGAU- -5' |
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| 2144 | 5' | -54.7 | NC_001362.1 | + | 2221 | 0.68 | 0.126423 |
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Target: 5'- gGgaGGCCUggGGUCgUGGuCCCCg- -3' miRNA: 3'- aCgaCUGGGuuUUAGgACCuGGGGau -5' |
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| 2144 | 5' | -54.7 | NC_001362.1 | + | 6803 | 0.69 | 0.107116 |
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Target: 5'- gUGCUGguuauGCCUAGug-UCUGGACCCCc- -3' miRNA: 3'- -ACGAC-----UGGGUUuuaGGACCUGGGGau -5' |
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| 2144 | 5' | -54.7 | NC_001362.1 | + | 3367 | 0.7 | 0.103602 |
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Target: 5'- gGCcgaGGCCCGAuAUCCgaGGuCCCCUAg -3' miRNA: 3'- aCGa--CUGGGUUuUAGGa-CCuGGGGAU- -5' |
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| 2144 | 5' | -54.7 | NC_001362.1 | + | 2333 | 1.07 | 0.000076 |
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Target: 5'- gUGCUGACCCAAAAUCCUGGACCCCUAa -3' miRNA: 3'- -ACGACUGGGUUUUAGGACCUGGGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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