Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21446 | 3' | -49.3 | NC_004812.1 | + | 114977 | 0.66 | 0.997848 |
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Target: 5'- gUCgGCCUCGGCgCUgacgUCAggGUGGAGg -3' miRNA: 3'- -AGgUGGAGCCG-GAaca-AGUa-CAUUUU- -5' |
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| 21446 | 3' | -49.3 | NC_004812.1 | + | 77107 | 0.66 | 0.995732 |
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Target: 5'- gCCGCCaUGGCCUUGgccugGUGGAGc -3' miRNA: 3'- aGGUGGaGCCGGAACaaguaCAUUUU- -5' |
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| 21446 | 3' | -49.3 | NC_004812.1 | + | 50469 | 0.67 | 0.994162 |
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Target: 5'- cUCgGCCUCGGCgg-GcgCGUGUGGAc -3' miRNA: 3'- -AGgUGGAGCCGgaaCaaGUACAUUUu -5' |
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| 21446 | 3' | -49.3 | NC_004812.1 | + | 92891 | 0.68 | 0.988167 |
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Target: 5'- aCCGCCUCGGCgacggcGUUCAgcuUGUAGc- -3' miRNA: 3'- aGGUGGAGCCGgaa---CAAGU---ACAUUuu -5' |
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| 21446 | 3' | -49.3 | NC_004812.1 | + | 149611 | 0.68 | 0.982758 |
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Target: 5'- cCCGCCgCGGCgaUGUUCGUGc---- -3' miRNA: 3'- aGGUGGaGCCGgaACAAGUACauuuu -5' |
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| 21446 | 3' | -49.3 | NC_004812.1 | + | 24102 | 0.68 | 0.982758 |
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Target: 5'- cCCGCCgCGGCgaUGUUCGUGc---- -3' miRNA: 3'- aGGUGGaGCCGgaACAAGUACauuuu -5' |
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| 21446 | 3' | -49.3 | NC_004812.1 | + | 13651 | 0.71 | 0.94679 |
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Target: 5'- gUCCACCcgggcaggUUGGCCggGUUCGUGaUGAAGc -3' miRNA: 3'- -AGGUGG--------AGCCGGaaCAAGUAC-AUUUU- -5' |
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| 21446 | 3' | -49.3 | NC_004812.1 | + | 63094 | 0.71 | 0.94679 |
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Target: 5'- cCCGCCUCGGCCUUcgUCAc------ -3' miRNA: 3'- aGGUGGAGCCGGAAcaAGUacauuuu -5' |
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| 21446 | 3' | -49.3 | NC_004812.1 | + | 93891 | 1.08 | 0.013443 |
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Target: 5'- gUCCACCUCGGCCUUGUUCAUGUAAAAg -3' miRNA: 3'- -AGGUGGAGCCGGAACAAGUACAUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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