Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2151 | 3' | -57.7 | NC_001362.1 | + | 6755 | 0.66 | 0.15296 |
Target: 5'- aGAUUUaguaaUCUGUCUCCCGUUCCUGCUg -3' miRNA: 3'- aCUAAAg----GGGCAGGGGGUGAGGGUGA- -5' |
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2151 | 3' | -57.7 | NC_001362.1 | + | 7297 | 0.72 | 0.04925 |
Target: 5'- cGGUagUUCCUGU-CCCUACUCCCGCg -3' miRNA: 3'- aCUAa-AGGGGCAgGGGGUGAGGGUGa -5' |
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2151 | 3' | -57.7 | NC_001362.1 | + | 6681 | 1.07 | 4.9e-05 |
Target: 5'- uUGAUUUCCCCGUCCCCCACUCCCACUc -3' miRNA: 3'- -ACUAAAGGGGCAGGGGGUGAGGGUGA- -5' |
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2151 | 3' | -57.7 | NC_001362.1 | + | 943 | 0.7 | 0.073136 |
Target: 5'- ---cUUCCCCcuucagCCCCCucuCUCCCACc -3' miRNA: 3'- acuaAAGGGGca----GGGGGu--GAGGGUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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