Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 21573 | 3' | -56 | NC_004812.1 | + | 136439 | 0.7 | 0.68618 |
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Target: 5'- gCUCGCCC-GGGUCgggGCCcGAGCg-- -3' miRNA: 3'- -GAGCGGGaCCCAGaa-UGGcUUCGaca -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 46785 | 0.71 | 0.676015 |
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Target: 5'- uCUCGCCCc-GGUCgaGCCGguGCUGg -3' miRNA: 3'- -GAGCGGGacCCAGaaUGGCuuCGACa -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 60736 | 0.71 | 0.676015 |
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Target: 5'- -cCGCUCgGGGUCgagACCGAGGCg-- -3' miRNA: 3'- gaGCGGGaCCCAGaa-UGGCUUCGaca -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 139081 | 0.71 | 0.644314 |
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Target: 5'- gCUgGCCCUGGGUUgcacggggaGCCGAaagacgcGGCUGg -3' miRNA: 3'- -GAgCGGGACCCAGaa-------UGGCU-------UCGACa -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 73968 | 0.72 | 0.602281 |
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Target: 5'- gUCGCCCUGGGcgUCgcucagggccaGCUGGAGCUGc -3' miRNA: 3'- gAGCGGGACCC--AGaa---------UGGCUUCGACa -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 86120 | 0.72 | 0.573758 |
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Target: 5'- aCUC-CCCUGGGUCaccGCCGAucgccguugcuGGCUGg -3' miRNA: 3'- -GAGcGGGACCCAGaa-UGGCU-----------UCGACa -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 13220 | 0.91 | 0.04581 |
|
Target: 5'- uCUCGCCCUGGGUCUU-CaCGAAGCUGUc -3' miRNA: 3'- -GAGCGGGACCCAGAAuG-GCUUCGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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