Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21573 | 3' | -56 | NC_004812.1 | + | 38540 | 0.67 | 0.867936 |
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Target: 5'- -gCGgCCgGGGUCgggGCgCGAAGCUGc -3' miRNA: 3'- gaGCgGGaCCCAGaa-UG-GCUUCGACa -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 38327 | 0.66 | 0.882416 |
|
Target: 5'- -gCGCCCgUGGG-CgaGCCGgcGCUGc -3' miRNA: 3'- gaGCGGG-ACCCaGaaUGGCuuCGACa -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 31192 | 0.66 | 0.889324 |
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Target: 5'- gUCGUCCgcaGGGUCgucccGCUGguGCUGUa -3' miRNA: 3'- gAGCGGGa--CCCAGaa---UGGCuuCGACA- -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 26409 | 0.66 | 0.914645 |
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Target: 5'- -cCGCCCgGGGcUCUggggcgcGCCGGAGgUGg -3' miRNA: 3'- gaGCGGGaCCC-AGAa------UGGCUUCgACa -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 13220 | 0.91 | 0.04581 |
|
Target: 5'- uCUCGCCCUGGGUCUU-CaCGAAGCUGUc -3' miRNA: 3'- -GAGCGGGACCCAGAAuG-GCUUCGACA- -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 9927 | 0.66 | 0.896004 |
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Target: 5'- -gCGCCC--GGUCUUggaACCGGAGCUc- -3' miRNA: 3'- gaGCGGGacCCAGAA---UGGCUUCGAca -5' |
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| 21573 | 3' | -56 | NC_004812.1 | + | 7639 | 0.67 | 0.867936 |
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Target: 5'- -gCGgCCgGGGUCgggGCgCGAAGCUGc -3' miRNA: 3'- gaGCgGGaCCCAGaa-UG-GCUUCGACa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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