Results 1 - 20 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21633 | 3' | -55.6 | NC_004812.1 | + | 2031 | 0.72 | 0.60238 |
Target: 5'- gCCGGcgggCCUCGGUCgGCGGCggGgGGCGc -3' miRNA: 3'- -GGUCa---GGAGUUAGaCGUCGa-CgCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 2090 | 0.7 | 0.724499 |
Target: 5'- aCCAGUCCUCGGg--GCGGCccggGCcGCGc -3' miRNA: 3'- -GGUCAGGAGUUagaCGUCGa---CGcCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 2126 | 0.7 | 0.73437 |
Target: 5'- cCCAGUCC-CAGUCcggggagGCcccgcgggcggGGCUcGCGGCGg -3' miRNA: 3'- -GGUCAGGaGUUAGa------CG-----------UCGA-CGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 4225 | 0.66 | 0.924998 |
Target: 5'- gUCGGg-CUCGGgg-GCGGCgGCGGCGg -3' miRNA: 3'- -GGUCagGAGUUagaCGUCGaCGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 5789 | 0.68 | 0.84324 |
Target: 5'- gUCGGcgcccgCCUCGggCUGCggGGCUGCGGgGc -3' miRNA: 3'- -GGUCa-----GGAGUuaGACG--UCGACGCCgC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 13257 | 0.66 | 0.929258 |
Target: 5'- aCCGGgaggCCUCcgacGUCgggGCGGCggaggccucggcGCGGCGg -3' miRNA: 3'- -GGUCa---GGAGu---UAGa--CGUCGa-----------CGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 16179 | 0.69 | 0.800447 |
Target: 5'- aCUGGgggCgCUCGAUCgccaGCAGCgcGCGGCGg -3' miRNA: 3'- -GGUCa--G-GAGUUAGa---CGUCGa-CGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 19668 | 0.71 | 0.653674 |
Target: 5'- -gGGUCCguggCGAUCUGCgcguucacguccAGCgcgGCGGCGc -3' miRNA: 3'- ggUCAGGa---GUUAGACG------------UCGa--CGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 21073 | 0.68 | 0.84324 |
Target: 5'- -gGGUCCg-AGUCcGCGGCggccgagGCGGCGa -3' miRNA: 3'- ggUCAGGagUUAGaCGUCGa------CGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 21909 | 0.67 | 0.881227 |
Target: 5'- cCCGGUUCgcgCAGcCgGCGGCU-CGGCGg -3' miRNA: 3'- -GGUCAGGa--GUUaGaCGUCGAcGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 26173 | 0.66 | 0.919455 |
Target: 5'- uCCAGcgccgCCUCcgcgggCcGCAGCgaGCGGCGg -3' miRNA: 3'- -GGUCa----GGAGuua---GaCGUCGa-CGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 27830 | 0.66 | 0.9303 |
Target: 5'- gCUGG-CCUCGcUCgccGCGcGCUGCGGCc -3' miRNA: 3'- -GGUCaGGAGUuAGa--CGU-CGACGCCGc -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 28469 | 0.71 | 0.704526 |
Target: 5'- cCCGGUCCgCGGUCcaucccgGCGGCggGCGGgGa -3' miRNA: 3'- -GGUCAGGaGUUAGa------CGUCGa-CGCCgC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 35449 | 0.78 | 0.329158 |
Target: 5'- cCCGGcCCUCGcagcgCUGCAGCgccGCGGCGu -3' miRNA: 3'- -GGUCaGGAGUua---GACGUCGa--CGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 36690 | 0.68 | 0.84324 |
Target: 5'- gUCGGcgcccgCCUCGggCUGCggGGCUGCGGgGc -3' miRNA: 3'- -GGUCa-----GGAGUuaGACG--UCGACGCCgC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 38358 | 0.68 | 0.826648 |
Target: 5'- uCCAGgaacUCCUC---CUGCAgGCggGCGGCGg -3' miRNA: 3'- -GGUC----AGGAGuuaGACGU-CGa-CGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 39138 | 0.67 | 0.894901 |
Target: 5'- gCCAGgCCUCGAUC-GCcuC-GCGGCGa -3' miRNA: 3'- -GGUCaGGAGUUAGaCGucGaCGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 41862 | 0.69 | 0.791396 |
Target: 5'- aCAGUCC-CGGgagaacgggCUGCGGCaGUGGCa -3' miRNA: 3'- gGUCAGGaGUUa--------GACGUCGaCGCCGc -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 43613 | 0.7 | 0.744152 |
Target: 5'- --cGUCCUCugccUCgGCGGCcGCGGCGc -3' miRNA: 3'- gguCAGGAGuu--AGaCGUCGaCGCCGC- -5' |
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21633 | 3' | -55.6 | NC_004812.1 | + | 44172 | 0.7 | 0.718537 |
Target: 5'- cUCGGgggCCgagcCGGUCUGCGGCggggguggggcucgGCGGCGg -3' miRNA: 3'- -GGUCa--GGa---GUUAGACGUCGa-------------CGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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