Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21633 | 3' | -55.6 | NC_004812.1 | + | 127599 | 0.7 | 0.724499 |
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Target: 5'- aCCAGUCCUCGGg--GCGGCccggGCcGCGc -3' miRNA: 3'- -GGUCAGGAGUUagaCGUCGa---CGcCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 127635 | 0.7 | 0.73437 |
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Target: 5'- cCCAGUCC-CAGUCcggggagGCcccgcgggcggGGCUcGCGGCGg -3' miRNA: 3'- -GGUCAGGaGUUAGa------CG-----------UCGA-CGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 136037 | 0.66 | 0.9303 |
|
Target: 5'- gCCGGUCCggucuggggaggUCcAUCaGCGGC-GCGGCc -3' miRNA: 3'- -GGUCAGG------------AGuUAGaCGUCGaCGCCGc -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 138696 | 0.66 | 0.924998 |
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Target: 5'- aCCGGgCCUCGcgCccggacgaccGCGGC-GCGGCGg -3' miRNA: 3'- -GGUCaGGAGUuaGa---------CGUCGaCGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 141560 | 0.66 | 0.907651 |
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Target: 5'- gCCAGUCCgccuccaGGUCccgGUgAGCgGCGGCGu -3' miRNA: 3'- -GGUCAGGag-----UUAGa--CG-UCGaCGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 151682 | 0.67 | 0.894901 |
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Target: 5'- uCCAGcgccgCCUCccgggCcGCAGCgaGCGGCGg -3' miRNA: 3'- -GGUCa----GGAGuua--GaCGUCGa-CGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 153338 | 0.66 | 0.9303 |
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Target: 5'- gCUGG-CCUCGcUCgccGCGcGCUGCGGCc -3' miRNA: 3'- -GGUCaGGAGUuAGa--CGU-CGACGCCGc -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 154014 | 0.7 | 0.724499 |
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Target: 5'- -gAGUCCggugCcGUCgGCGGCgGCGGCGu -3' miRNA: 3'- ggUCAGGa---GuUAGaCGUCGaCGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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