Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21633 | 3' | -55.6 | NC_004812.1 | + | 19668 | 0.71 | 0.653674 |
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Target: 5'- -gGGUCCguggCGAUCUGCgcguucacguccAGCgcgGCGGCGc -3' miRNA: 3'- ggUCAGGa---GUUAGACG------------UCGa--CGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 16179 | 0.69 | 0.800447 |
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Target: 5'- aCUGGgggCgCUCGAUCgccaGCAGCgcGCGGCGg -3' miRNA: 3'- -GGUCa--G-GAGUUAGa---CGUCGa-CGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 13257 | 0.66 | 0.929258 |
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Target: 5'- aCCGGgaggCCUCcgacGUCgggGCGGCggaggccucggcGCGGCGg -3' miRNA: 3'- -GGUCa---GGAGu---UAGa--CGUCGa-----------CGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 5789 | 0.68 | 0.84324 |
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Target: 5'- gUCGGcgcccgCCUCGggCUGCggGGCUGCGGgGc -3' miRNA: 3'- -GGUCa-----GGAGUuaGACG--UCGACGCCgC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 4225 | 0.66 | 0.924998 |
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Target: 5'- gUCGGg-CUCGGgg-GCGGCgGCGGCGg -3' miRNA: 3'- -GGUCagGAGUUagaCGUCGaCGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 2126 | 0.7 | 0.73437 |
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Target: 5'- cCCAGUCC-CAGUCcggggagGCcccgcgggcggGGCUcGCGGCGg -3' miRNA: 3'- -GGUCAGGaGUUAGa------CG-----------UCGA-CGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 2090 | 0.7 | 0.724499 |
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Target: 5'- aCCAGUCCUCGGg--GCGGCccggGCcGCGc -3' miRNA: 3'- -GGUCAGGAGUUagaCGUCGa---CGcCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 2031 | 0.72 | 0.60238 |
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Target: 5'- gCCGGcgggCCUCGGUCgGCGGCggGgGGCGc -3' miRNA: 3'- -GGUCa---GGAGUUAGaCGUCGa-CgCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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