Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21633 | 3' | -55.6 | NC_004812.1 | + | 41862 | 0.69 | 0.791396 |
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Target: 5'- aCAGUCC-CGGgagaacgggCUGCGGCaGUGGCa -3' miRNA: 3'- gGUCAGGaGUUa--------GACGUCGaCGCCGc -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 119471 | 0.69 | 0.809346 |
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Target: 5'- aCGGcCCUCc-UCUGC-GCcGCGGCGg -3' miRNA: 3'- gGUCaGGAGuuAGACGuCGaCGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 87027 | 0.68 | 0.818082 |
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Target: 5'- aCCGccGUCgcgCUCGGag-GCGGCUGCGGCGc -3' miRNA: 3'- -GGU--CAG---GAGUUagaCGUCGACGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 126914 | 0.68 | 0.835037 |
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Target: 5'- cCCAGUCgUCGugcGUCccugGCGGUUucucGCGGCGu -3' miRNA: 3'- -GGUCAGgAGU---UAGa---CGUCGA----CGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 21073 | 0.68 | 0.84324 |
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Target: 5'- -gGGUCCg-AGUCcGCGGCggccgagGCGGCGa -3' miRNA: 3'- ggUCAGGagUUAGaCGUCGa------CGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 36690 | 0.68 | 0.84324 |
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Target: 5'- gUCGGcgcccgCCUCGggCUGCggGGCUGCGGgGc -3' miRNA: 3'- -GGUCa-----GGAGUuaGACG--UCGACGCCgC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 121878 | 0.68 | 0.851249 |
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Target: 5'- cCCAGgacggCGAUCgGCGGCUGcCGGUGu -3' miRNA: 3'- -GGUCagga-GUUAGaCGUCGAC-GCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 84686 | 0.77 | 0.360056 |
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Target: 5'- gCC-GUCCUCGccgCUGCAGCgcccGCGGCGc -3' miRNA: 3'- -GGuCAGGAGUua-GACGUCGa---CGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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