Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21633 | 3' | -55.6 | NC_004812.1 | + | 103193 | 0.67 | 0.894239 |
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Target: 5'- cCCGGgcUCCUCGGUggccgcgCUGCGGacgaGCGGCa -3' miRNA: 3'- -GGUC--AGGAGUUA-------GACGUCga--CGCCGc -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 109466 | 0.67 | 0.888178 |
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Target: 5'- gCGGgggcgCCcgCGggCUGCGGCaucgUGCGGCGg -3' miRNA: 3'- gGUCa----GGa-GUuaGACGUCG----ACGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 21909 | 0.67 | 0.881227 |
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Target: 5'- cCCGGUUCgcgCAGcCgGCGGCU-CGGCGg -3' miRNA: 3'- -GGUCAGGa--GUUaGaCGUCGAcGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 126744 | 0.67 | 0.866663 |
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Target: 5'- -gGGUCCUgGcggacggcuuGUCUGCGGCUGaCuGCGa -3' miRNA: 3'- ggUCAGGAgU----------UAGACGUCGAC-GcCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 100776 | 0.67 | 0.859059 |
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Target: 5'- gCAGUCgC-CGAUCgUGCGGUgGCGGCc -3' miRNA: 3'- gGUCAG-GaGUUAG-ACGUCGaCGCCGc -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 98731 | 0.68 | 0.851249 |
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Target: 5'- --cGUUCUCGcgCggggGCAGCgcccccgGCGGCGa -3' miRNA: 3'- gguCAGGAGUuaGa---CGUCGa------CGCCGC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 5789 | 0.68 | 0.84324 |
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Target: 5'- gUCGGcgcccgCCUCGggCUGCggGGCUGCGGgGc -3' miRNA: 3'- -GGUCa-----GGAGUuaGACG--UCGACGCCgC- -5' |
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| 21633 | 3' | -55.6 | NC_004812.1 | + | 57932 | 1.1 | 0.002623 |
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Target: 5'- gCCAGUCCUCAAUCUGCAGCUGCGGCGu -3' miRNA: 3'- -GGUCAGGAGUUAGACGUCGACGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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