miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2164 5' -55.1 NC_001363.1 + 5483 1.08 0.000034
Target:  5'- gGGGCCAAGAACAGAUGGUCCCCAGAUg -3'
miRNA:   3'- -CCCGGUUCUUGUCUACCAGGGGUCUA- -5'
2164 5' -55.1 NC_001363.1 + 5407 0.69 0.081526
Target:  5'- aGGGCCAAGAACAGAUGagacagcugagugauGggCCaaaCAGGa -3'
miRNA:   3'- -CCCGGUUCUUGUCUAC---------------Ca-GGg--GUCUa -5'
2164 5' -55.1 NC_001363.1 + 4110 0.75 0.026167
Target:  5'- cGGGagcCCGAGGAgGaGUGGUCCCCAGGa -3'
miRNA:   3'- -CCC---GGUUCUUgUcUACCAGGGGUCUa -5'
2164 5' -55.1 NC_001363.1 + 2651 0.68 0.086142
Target:  5'- aGGGUCAGGAG-GGAggucuggggucuUGGUCCCCgAGGUc -3'
miRNA:   3'- -CCCGGUUCUUgUCU------------ACCAGGGG-UCUA- -5'
2164 5' -55.1 NC_001363.1 + 245 1.08 0.000034
Target:  5'- gGGGCCAAGAACAGAUGGUCCCCAGAUg -3'
miRNA:   3'- -CCCGGUUCUUGUCUACCAGGGGUCUA- -5'
2164 5' -55.1 NC_001363.1 + 169 0.69 0.081526
Target:  5'- aGGGCCAAGAACAGAUGagacagcugagugauGggCCaaaCAGGa -3'
miRNA:   3'- -CCCGGUUCUUGUCUAC---------------Ca-GGg--GUCUa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.