Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21701 | 3' | -50.3 | NC_004812.1 | + | 110369 | 0.66 | 0.995459 |
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Target: 5'- uCGAGGAcACGAUCUucGC-GCU-CUUCUg -3' miRNA: 3'- -GCUCCU-UGCUAGG--UGaCGAaGAAGAa -5' |
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| 21701 | 3' | -50.3 | NC_004812.1 | + | 84171 | 0.66 | 0.994698 |
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Target: 5'- gCGGGGAGCGAUCCAacgGCg------- -3' miRNA: 3'- -GCUCCUUGCUAGGUga-CGaagaagaa -5' |
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| 21701 | 3' | -50.3 | NC_004812.1 | + | 53523 | 0.66 | 0.993836 |
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Target: 5'- gGAGGAagauGCGGUCCACgGCcUC-UCg- -3' miRNA: 3'- gCUCCU----UGCUAGGUGaCGaAGaAGaa -5' |
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| 21701 | 3' | -50.3 | NC_004812.1 | + | 55594 | 0.67 | 0.987707 |
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Target: 5'- cCGAGG--CGAUCCGCUGCcUCg---- -3' miRNA: 3'- -GCUCCuuGCUAGGUGACGaAGaagaa -5' |
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| 21701 | 3' | -50.3 | NC_004812.1 | + | 111039 | 0.68 | 0.982231 |
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Target: 5'- gCGAGGAACGG-CCugUGCgugUCg---- -3' miRNA: 3'- -GCUCCUUGCUaGGugACGa--AGaagaa -5' |
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| 21701 | 3' | -50.3 | NC_004812.1 | + | 69363 | 0.74 | 0.785575 |
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Target: 5'- gCGAGcauGugGAUCCACUGCUUCgUUCg- -3' miRNA: 3'- -GCUCc--UugCUAGGUGACGAAG-AAGaa -5' |
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| 21701 | 3' | -50.3 | NC_004812.1 | + | 114488 | 1.06 | 0.014397 |
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Target: 5'- aCGAGGAACGAUCCACUGCUUCUUCUUc -3' miRNA: 3'- -GCUCCUUGCUAGGUGACGAAGAAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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