miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21733 3' -52.7 NC_004813.1 + 12788 0.66 0.83161
Target:  5'- cUUUCCCGGcGAGucuggcuaaCCGGGACCagaacgaACUGa -3'
miRNA:   3'- aAAAGGGCCuUUC---------GGUCUUGGg------UGAC- -5'
21733 3' -52.7 NC_004813.1 + 5057 0.66 0.822238
Target:  5'- gUUUUCCCc--AGGCCAGAGCCUuaGCg- -3'
miRNA:   3'- -AAAAGGGccuUUCGGUCUUGGG--UGac -5'
21733 3' -52.7 NC_004813.1 + 28500 0.66 0.792888
Target:  5'- --aUCCCGGc-AGCCAGcgugGACUUGCUGu -3'
miRNA:   3'- aaaAGGGCCuuUCGGUC----UUGGGUGAC- -5'
21733 3' -52.7 NC_004813.1 + 43066 0.66 0.792888
Target:  5'- ---cCCCGGcaguGAAGaaAGAGCCCACg- -3'
miRNA:   3'- aaaaGGGCC----UUUCggUCUUGGGUGac -5'
21733 3' -52.7 NC_004813.1 + 27633 0.68 0.718735
Target:  5'- --cUCCCGGGu--CCAGAACgCACUc -3'
miRNA:   3'- aaaAGGGCCUuucGGUCUUGgGUGAc -5'
21733 3' -52.7 NC_004813.1 + 48536 0.68 0.717633
Target:  5'- ---aCCCGGAccaaaggGAGaaCCGGGGCCCGCg- -3'
miRNA:   3'- aaaaGGGCCU-------UUC--GGUCUUGGGUGac -5'
21733 3' -52.7 NC_004813.1 + 50402 0.69 0.674074
Target:  5'- --gUgCCGGAcGGCCGacGGGCCCACa- -3'
miRNA:   3'- aaaAgGGCCUuUCGGU--CUUGGGUGac -5'
21733 3' -52.7 NC_004813.1 + 9664 0.69 0.628733
Target:  5'- -gUUCCCuauGGAcAAGCCAGAuCCCAUa- -3'
miRNA:   3'- aaAAGGG---CCU-UUCGGUCUuGGGUGac -5'
21733 3' -52.7 NC_004813.1 + 39313 0.69 0.628733
Target:  5'- ---gCCCGGGuucGCCAGAACaUACUGa -3'
miRNA:   3'- aaaaGGGCCUuu-CGGUCUUGgGUGAC- -5'
21733 3' -52.7 NC_004813.1 + 45975 0.7 0.561008
Target:  5'- -cUUCCCGGGcuuaaugugcGGCCGGAuacACuCCACUGa -3'
miRNA:   3'- aaAAGGGCCUu---------UCGGUCU---UG-GGUGAC- -5'
21733 3' -52.7 NC_004813.1 + 12873 0.72 0.489055
Target:  5'- --gUCCCGGuuAGCCAGAcucgccgggaaaguuGCCaguaACUGg -3'
miRNA:   3'- aaaAGGGCCuuUCGGUCU---------------UGGg---UGAC- -5'
21733 3' -52.7 NC_004813.1 + 12927 1.07 0.002124
Target:  5'- gUUUUCCCGGAAAGCCAGAACCCACUGg -3'
miRNA:   3'- -AAAAGGGCCUUUCGGUCUUGGGUGAC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.