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miRNA: 3'- aUUCGagu---CAGUa---CGACCGGA--AGGCG- -5'
miRNA: 3'- auUCGAGU--CAGU--ACGACCGGa---------AGGCG- -5'
miRNA: 3'- -AUUCGAGUCAGUACGACCGGAAGGCG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 21734 | 3' | -54.1 | NC_004813.1 | + | 38631 | 0.68 | 0.648591 |
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Target: 5'- aGAGCguuuuuGUCAccccGCUGGCCUcaUUCGCa -3' miRNA: 3'- aUUCGagu---CAGUa---CGACCGGA--AGGCG- -5' |
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| 21734 | 3' | -54.1 | NC_004813.1 | + | 44341 | 0.7 | 0.515172 |
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Target: 5'- -uAGUUCAccGUCAucUGCUGGCCaccaaacugcUCCGCa -3' miRNA: 3'- auUCGAGU--CAGU--ACGACCGGa---------AGGCG- -5' |
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| 21734 | 3' | -54.1 | NC_004813.1 | + | 14929 | 1.11 | 0.000755 |
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Target: 5'- aUAAGCUCAGUCAUGCUGGCCUUCCGCa -3' miRNA: 3'- -AUUCGAGUCAGUACGACCGGAAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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