Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21735 | 3' | -50 | NC_004813.1 | + | 30181 | 0.66 | 0.935881 |
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Target: 5'- uCAggGGGCuGuCCGUGggcuucCGUCCUCAu -3' miRNA: 3'- -GUuaCCCG-CuGGUACauu---GUAGGAGU- -5' |
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| 21735 | 3' | -50 | NC_004813.1 | + | 12433 | 0.67 | 0.924299 |
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Target: 5'- uGAUGGcGCGAuagucuucaCCAUGUcaAACAUCCa-- -3' miRNA: 3'- gUUACC-CGCU---------GGUACA--UUGUAGGagu -5' |
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| 21735 | 3' | -50 | NC_004813.1 | + | 33532 | 0.68 | 0.882423 |
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Target: 5'- ---cGGGCGAUgAUGU-GCGUCCg-- -3' miRNA: 3'- guuaCCCGCUGgUACAuUGUAGGagu -5' |
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| 21735 | 3' | -50 | NC_004813.1 | + | 36022 | 0.7 | 0.801497 |
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Target: 5'- uGAUGGGCGGgCAggcggaGUCCUCAg -3' miRNA: 3'- gUUACCCGCUgGUacauugUAGGAGU- -5' |
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| 21735 | 3' | -50 | NC_004813.1 | + | 38781 | 0.7 | 0.791396 |
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Target: 5'- ---aGGGCaGCCGUGUAcCGUgCUCAg -3' miRNA: 3'- guuaCCCGcUGGUACAUuGUAgGAGU- -5' |
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| 21735 | 3' | -50 | NC_004813.1 | + | 18525 | 1.09 | 0.003473 |
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Target: 5'- gCAAUGGGCGACCAUGUAACAUCCUCAu -3' miRNA: 3'- -GUUACCCGCUGGUACAUUGUAGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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