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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21735 | 5' | -62.1 | NC_004813.1 | + | 14539 | 0.72 | 0.134584 |
|
Target: 5'- uGgUGCCCAUGCCCCCCuG-GCUUcacaGAGg -3' miRNA: 3'- -CgGCGGGUACGGGGGGuCgCGAG----UUC- -5' |
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| 21735 | 5' | -62.1 | NC_004813.1 | + | 18490 | 1.1 | 0.00019 |
|
Target: 5'- uGCCGCCCAUGCCCCCCAGCGCUCAAGa -3' miRNA: 3'- -CGGCGGGUACGGGGGGUCGCGAGUUC- -5' |
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| 21735 | 5' | -62.1 | NC_004813.1 | + | 22281 | 0.66 | 0.34182 |
|
Target: 5'- -gCGCCCGUcaucGCCUUCCGGUGCagAAGc -3' miRNA: 3'- cgGCGGGUA----CGGGGGGUCGCGagUUC- -5' |
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| 21735 | 5' | -62.1 | NC_004813.1 | + | 30221 | 0.66 | 0.374493 |
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Target: 5'- gGCCGCCCAgGCCUcaUCCAGC-CUg--- -3' miRNA: 3'- -CGGCGGGUaCGGG--GGGUCGcGAguuc -5' |
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| 21735 | 5' | -62.1 | NC_004813.1 | + | 35371 | 0.66 | 0.391605 |
|
Target: 5'- gGCUGCCCGUGCCggaugcgaCCGGCGggCGu- -3' miRNA: 3'- -CGGCGGGUACGGgg------GGUCGCgaGUuc -5' |
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| 21735 | 5' | -62.1 | NC_004813.1 | + | 43861 | 0.7 | 0.20984 |
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Target: 5'- uCUGCUCcUGCUCCCCGGCGCgcCAGa -3' miRNA: 3'- cGGCGGGuACGGGGGGUCGCGa-GUUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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