miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21740 5' -51.6 NC_004813.1 + 48247 0.66 0.845157
Target:  5'- cGGAGUCaGCCcggcAGgcgGCAgaaaGUGCAGCCUc -3'
miRNA:   3'- uUUUUAG-CGG----UCa--CGUa---CACGUCGGA- -5'
21740 5' -51.6 NC_004813.1 + 45983 0.66 0.845157
Target:  5'- cAGAAAUCcuucCCGG-GCuuaAUGUGCGGCCg -3'
miRNA:   3'- -UUUUUAGc---GGUCaCG---UACACGUCGGa -5'
21740 5' -51.6 NC_004813.1 + 31256 0.66 0.826462
Target:  5'- ------gGaCCAGUGCAUcaGCAGCCg -3'
miRNA:   3'- uuuuuagC-GGUCACGUAcaCGUCGGa -5'
21740 5' -51.6 NC_004813.1 + 32585 0.69 0.675798
Target:  5'- -----cCGCCGGUGUAUGccgccgggcugGCGGCCa -3'
miRNA:   3'- uuuuuaGCGGUCACGUACa----------CGUCGGa -5'
21740 5' -51.6 NC_004813.1 + 3100 0.69 0.663109
Target:  5'- ---cGUCGCCAGuUGCcugccauucgauaAUG-GCAGCCUg -3'
miRNA:   3'- uuuuUAGCGGUC-ACG-------------UACaCGUCGGA- -5'
21740 5' -51.6 NC_004813.1 + 38796 0.75 0.345495
Target:  5'- -----cCGCCAGUGCAacuucauuacGUGCAGCCg -3'
miRNA:   3'- uuuuuaGCGGUCACGUa---------CACGUCGGa -5'
21740 5' -51.6 NC_004813.1 + 28316 1.06 0.002729
Target:  5'- uAAAAAUCGCCAGUGCAUGUGCAGCCUg -3'
miRNA:   3'- -UUUUUAGCGGUCACGUACACGUCGGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.