Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21743 | 3' | -62.8 | NC_004813.1 | + | 32092 | 0.66 | 0.360893 |
Target: 5'- uCAGgCGGUGGCugaGCCCuuugccGGCGCCUGg -3' miRNA: 3'- -GUCgGCCGUCGuggCGGG------CUGUGGAC- -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 44563 | 0.66 | 0.350372 |
Target: 5'- uGGUC-GCAGUACUGCCCGAuggcauacagcgccCACUUGu -3' miRNA: 3'- gUCGGcCGUCGUGGCGGGCU--------------GUGGAC- -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 33359 | 0.66 | 0.352781 |
Target: 5'- uCAGa-GGCAGCgACCGCCUGAgGCa-- -3' miRNA: 3'- -GUCggCCGUCG-UGGCGGGCUgUGgac -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 34924 | 0.67 | 0.285658 |
Target: 5'- aGGCCaGCAGCACggcgguaGCCUGACgaACCUu -3' miRNA: 3'- gUCGGcCGUCGUGg------CGGGCUG--UGGAc -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 21482 | 0.67 | 0.285658 |
Target: 5'- -uGCauGCuGCACCGCCCGGuguCACCg- -3' miRNA: 3'- guCGgcCGuCGUGGCGGGCU---GUGGac -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 27212 | 0.67 | 0.314177 |
Target: 5'- gCGGCgGGUGGUauGCCGgaCCCGACGCUg- -3' miRNA: 3'- -GUCGgCCGUCG--UGGC--GGGCUGUGGac -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 45114 | 0.67 | 0.30685 |
Target: 5'- -uGCCGGagacCGGUGCCGCCaCGGugaACCUGa -3' miRNA: 3'- guCGGCC----GUCGUGGCGG-GCUg--UGGAC- -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 33991 | 0.67 | 0.284288 |
Target: 5'- gCAGCCGGCGGacggcaaaagucuCACCccggaugaugugcGUCCGAUGCUUGa -3' miRNA: 3'- -GUCGGCCGUC-------------GUGG-------------CGGGCUGUGGAC- -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 37867 | 0.67 | 0.272182 |
Target: 5'- gCAGCCaGGgAGCAgCGCCgGGC-UCUGg -3' miRNA: 3'- -GUCGG-CCgUCGUgGCGGgCUGuGGAC- -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 33830 | 0.68 | 0.264345 |
Target: 5'- aCAGCCGGCGGacgguaaaagucuCACCccggaugaugugcGUCCGAUGCUUGa -3' miRNA: 3'- -GUCGGCCGUC-------------GUGG-------------CGGGCUGUGGAC- -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 33668 | 0.68 | 0.264345 |
Target: 5'- aCAGCCGGCGGacgguaaaagucuCACCccggaugaugugcGUCCGAUGCUUGa -3' miRNA: 3'- -GUCGGCCGUC-------------GUGG-------------CGGGCUGUGGAC- -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 34153 | 0.68 | 0.239538 |
Target: 5'- gCAGCCGGCAGacgguaaaaguauCACCccggaugaugugcGUCCGAUGCUUGa -3' miRNA: 3'- -GUCGGCCGUC-------------GUGG-------------CGGGCUGUGGAC- -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 37967 | 0.68 | 0.240731 |
Target: 5'- uGGCUGuGCAGCucaGCCagaGCCCGGCGCUg- -3' miRNA: 3'- gUCGGC-CGUCG---UGG---CGGGCUGUGGac -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 27139 | 0.69 | 0.21237 |
Target: 5'- aCAGa-GaCAGCACUGCCCGGCAucCCUGa -3' miRNA: 3'- -GUCggCcGUCGUGGCGGGCUGU--GGAC- -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 30631 | 0.69 | 0.229024 |
Target: 5'- aAGCCGGUAagGCCGCCaCGGCGCa-- -3' miRNA: 3'- gUCGGCCGUcgUGGCGG-GCUGUGgac -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 21379 | 0.71 | 0.159978 |
Target: 5'- gCAGCUGGC-GCGCCGCaguacggUGACACCg- -3' miRNA: 3'- -GUCGGCCGuCGUGGCGg------GCUGUGGac -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 23573 | 0.72 | 0.14025 |
Target: 5'- uCAGUCGGUGGCuCUGCCUGuACACCa- -3' miRNA: 3'- -GUCGGCCGUCGuGGCGGGC-UGUGGac -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 39236 | 0.73 | 0.107339 |
Target: 5'- uCAGCCGGUucugcucugucAGCGCCGCCCGuguuCGuCCUu -3' miRNA: 3'- -GUCGGCCG-----------UCGUGGCGGGCu---GU-GGAc -5' |
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21743 | 3' | -62.8 | NC_004813.1 | + | 31117 | 1.08 | 0.00023 |
Target: 5'- cCAGCCGGCAGCACCGCCCGACACCUGg -3' miRNA: 3'- -GUCGGCCGUCGUGGCGGGCUGUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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