miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21745 5' -54.2 NC_004813.1 + 35690 0.69 0.577669
Target:  5'- -aGCGCCGggaaaGGACCACcacauugCGGCGCAg- -3'
miRNA:   3'- agUGCGGUa----CCUGGUGa------GUCGUGUac -5'
21745 5' -54.2 NC_004813.1 + 35408 1.09 0.001126
Target:  5'- gUCACGCCAUGGACCACUCAGCACAUGa -3'
miRNA:   3'- -AGUGCGGUACCUGGUGAGUCGUGUAC- -5'
21745 5' -54.2 NC_004813.1 + 33056 0.67 0.720431
Target:  5'- cCACGCUgcugagaAUGGACagugagCGGCGCAUGa -3'
miRNA:   3'- aGUGCGG-------UACCUGguga--GUCGUGUAC- -5'
21745 5' -54.2 NC_004813.1 + 31288 0.66 0.784202
Target:  5'- aCGCGCCAcc-ACCGCUuccgcCAGCGCAUu -3'
miRNA:   3'- aGUGCGGUaccUGGUGA-----GUCGUGUAc -5'
21745 5' -54.2 NC_004813.1 + 15187 0.69 0.588727
Target:  5'- aUCAgGCCGUGGguggaaaaaGCCGCUgagCAGUGCAUa -3'
miRNA:   3'- -AGUgCGGUACC---------UGGUGA---GUCGUGUAc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.