Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21748 | 5' | -50.8 | NC_004813.1 | + | 38555 | 1.12 | 0.001893 |
Target: 5'- aCUACUGGAAACCGUUUCCGGCAUGGCc -3' miRNA: 3'- -GAUGACCUUUGGCAAAGGCCGUACCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 32075 | 0.73 | 0.575087 |
Target: 5'- -gGCUGGAccAGCCuGUUUCaGGCgGUGGCu -3' miRNA: 3'- gaUGACCU--UUGG-CAAAGgCCG-UACCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 35948 | 0.72 | 0.642667 |
Target: 5'- cCUGCUGGAuggcaGGCCGUcgguggcgcUUCCGuGgGUGGUu -3' miRNA: 3'- -GAUGACCU-----UUGGCA---------AAGGC-CgUACCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 30625 | 0.71 | 0.698784 |
Target: 5'- -aACUGGAAGCCGguaaggCCGcCAcGGCg -3' miRNA: 3'- gaUGACCUUUGGCaaa---GGCcGUaCCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 21131 | 0.71 | 0.720836 |
Target: 5'- -gGCUGGcAGCCGUUugguagUCCGGU--GGCc -3' miRNA: 3'- gaUGACCuUUGGCAA------AGGCCGuaCCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 38632 | 0.7 | 0.753188 |
Target: 5'- -cGCUGGuuGCCGUcgggguugcCCGGUAcuuUGGCa -3' miRNA: 3'- gaUGACCuuUGGCAaa-------GGCCGU---ACCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 703 | 0.69 | 0.814052 |
Target: 5'- -cACgGGGAGCUGguaucgCUGGCAUGGg -3' miRNA: 3'- gaUGaCCUUUGGCaaa---GGCCGUACCg -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 41144 | 0.68 | 0.832854 |
Target: 5'- -gGCUGGAAGa-GUUUUuaucgUGGCAUGGUg -3' miRNA: 3'- gaUGACCUUUggCAAAG-----GCCGUACCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 6118 | 0.67 | 0.889688 |
Target: 5'- -gGCUGgGAAGCUGgcugaacgugUCGGCAUGGa -3' miRNA: 3'- gaUGAC-CUUUGGCaaa-------GGCCGUACCg -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 31473 | 0.67 | 0.905444 |
Target: 5'- gUGCUGGAcaucagccaGACCGca-CCGGUG-GGCu -3' miRNA: 3'- gAUGACCU---------UUGGCaaaGGCCGUaCCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 16506 | 0.67 | 0.8758 |
Target: 5'- aUACU-GAGGCCGgaagcggggauUUUCCGGCG-GGUa -3' miRNA: 3'- gAUGAcCUUUGGC-----------AAAGGCCGUaCCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 41439 | 0.67 | 0.8758 |
Target: 5'- ----aGGGAGCCGgugacCUGGCAggGGCg -3' miRNA: 3'- gaugaCCUUUGGCaaa--GGCCGUa-CCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 39411 | 0.67 | 0.904758 |
Target: 5'- -gGCUGGaAGACCGUcgUgUGGCGUuaauucgucagcaGGCg -3' miRNA: 3'- gaUGACC-UUUGGCAa-AgGCCGUA-------------CCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 30079 | 0.67 | 0.898451 |
Target: 5'- -gGCUGGAAAUCa----CGGCAacacUGGCg -3' miRNA: 3'- gaUGACCUUUGGcaaagGCCGU----ACCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 45112 | 0.67 | 0.898451 |
Target: 5'- aCUGCcGGAGACCGgugCCGcCAcGGUg -3' miRNA: 3'- -GAUGaCCUUUGGCaaaGGCcGUaCCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 53418 | 0.66 | 0.92939 |
Target: 5'- uUGCUGGAAagGCUGggccacacuccUCCGGCAgaaGCa -3' miRNA: 3'- gAUGACCUU--UGGCaa---------AGGCCGUac-CG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 30200 | 0.66 | 0.924697 |
Target: 5'- --cCUGGcAGCCaGUUgcgauggcauaUCCGGCAcgGGCu -3' miRNA: 3'- gauGACCuUUGG-CAA-----------AGGCCGUa-CCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 31743 | 0.66 | 0.930534 |
Target: 5'- -aACaUGGAAaccagcuccACCGgcgaCGGCGUGGUa -3' miRNA: 3'- gaUG-ACCUU---------UGGCaaagGCCGUACCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 49673 | 0.66 | 0.938216 |
Target: 5'- aUGCUGGcGAACCcagacacgcagaGUcUCCGGCGUacagccaaucuuuggGGCa -3' miRNA: 3'- gAUGACC-UUUGG------------CAaAGGCCGUA---------------CCG- -5' |
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21748 | 5' | -50.8 | NC_004813.1 | + | 50399 | 0.66 | 0.934446 |
Target: 5'- -gGCUuaaGGAAGCCGgauuaCUGGCAucaacagggaguacUGGCg -3' miRNA: 3'- gaUGA---CCUUUGGCaaa--GGCCGU--------------ACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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