Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21749 | 3' | -59.9 | NC_004813.1 | + | 24330 | 0.66 | 0.445723 |
Target: 5'- gUCGGCuGcCGUUCugGcGCUGaUUGCCGCAg -3' miRNA: 3'- -GGUCG-C-GCGGGugC-CGAC-AACGGUGU- -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 25979 | 0.68 | 0.364327 |
Target: 5'- -gAGCGCGUugUCACGGUaaugguuUGUUGCCAg- -3' miRNA: 3'- ggUCGCGCG--GGUGCCG-------ACAACGGUgu -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 30604 | 0.66 | 0.463967 |
Target: 5'- uCCAGCguGCGCCCuuccucuGCGGCUuugGUCAUg -3' miRNA: 3'- -GGUCG--CGCGGG-------UGCCGAcaaCGGUGu -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 31781 | 0.69 | 0.332732 |
Target: 5'- uCCAGCGCuCCgCACGGaug--GCCACGc -3' miRNA: 3'- -GGUCGCGcGG-GUGCCgacaaCGGUGU- -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 31782 | 0.69 | 0.332732 |
Target: 5'- gCAGUGCGUCCgGCGGCUGa-GUCugAu -3' miRNA: 3'- gGUCGCGCGGG-UGCCGACaaCGGugU- -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 31990 | 0.76 | 0.103744 |
Target: 5'- aCCAGCacucCGCaCACGGCUG-UGCCACAu -3' miRNA: 3'- -GGUCGc---GCGgGUGCCGACaACGGUGU- -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 37944 | 0.66 | 0.474706 |
Target: 5'- aCCGGUGCGUuuGcCGGUgccuUUGCCACc -3' miRNA: 3'- -GGUCGCGCGggU-GCCGac--AACGGUGu -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 37945 | 0.67 | 0.408673 |
Target: 5'- cCCGGCGCuGCuCCcUGGC---UGCCGCAg -3' miRNA: 3'- -GGUCGCG-CG-GGuGCCGacaACGGUGU- -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 38695 | 1.1 | 0.000311 |
Target: 5'- aCCAGCGCGCCCACGGCUGUUGCCACAu -3' miRNA: 3'- -GGUCGCGCGGGUGCCGACAACGGUGU- -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 41847 | 0.66 | 0.494542 |
Target: 5'- aCAGCGCaccGUCCGucuCGGUcggUGUUGCCAgCAc -3' miRNA: 3'- gGUCGCG---CGGGU---GCCG---ACAACGGU-GU- -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 43848 | 0.66 | 0.483584 |
Target: 5'- cCCGGCGCGCCagACcGCUGUcacaccaUGUauCACAg -3' miRNA: 3'- -GGUCGCGCGGg-UGcCGACA-------ACG--GUGU- -5' |
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21749 | 3' | -59.9 | NC_004813.1 | + | 46480 | 0.68 | 0.348679 |
Target: 5'- aCAGCugauuccccuGCGCCacaaauaACGGCgugcUGUUGCCGCu -3' miRNA: 3'- gGUCG----------CGCGGg------UGCCG----ACAACGGUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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