Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21751 | 3' | -58.8 | NC_004813.1 | + | 48236 | 0.66 | 0.484416 |
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Target: 5'- -aGGGGAggcaucggagUCAGCCCggca-GGCGGCa -3' miRNA: 3'- aaCUCCUa---------AGUCGGGacaccCCGCCG- -5' |
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| 21751 | 3' | -58.8 | NC_004813.1 | + | 48600 | 0.67 | 0.435238 |
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Target: 5'- -cGuGGugacacCGGCCCUGUcGGGGCaaccGGCg -3' miRNA: 3'- aaCuCCuaa---GUCGGGACA-CCCCG----CCG- -5' |
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| 21751 | 3' | -58.8 | NC_004813.1 | + | 37156 | 0.67 | 0.425752 |
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Target: 5'- -aGAGGAUgacgauaCCCUGUcGGuGGUGGCg -3' miRNA: 3'- aaCUCCUAaguc---GGGACA-CC-CCGCCG- -5' |
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| 21751 | 3' | -58.8 | NC_004813.1 | + | 41439 | 0.69 | 0.346235 |
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Target: 5'- --aGGGAgcCGGUgaCCUGgcaGGGGCGGCa -3' miRNA: 3'- aacUCCUaaGUCG--GGACa--CCCCGCCG- -5' |
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| 21751 | 3' | -58.8 | NC_004813.1 | + | 30028 | 0.69 | 0.322202 |
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Target: 5'- aUGAccAUUCccGCCCUGUGGGGCaGUg -3' miRNA: 3'- aACUccUAAGu-CGGGACACCCCGcCG- -5' |
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| 21751 | 3' | -58.8 | NC_004813.1 | + | 43023 | 0.7 | 0.284964 |
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Target: 5'- aUG-GGGUUCAGCCCUGgcagccGGUGGUu -3' miRNA: 3'- aACuCCUAAGUCGGGACacc---CCGCCG- -5' |
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| 21751 | 3' | -58.8 | NC_004813.1 | + | 41149 | 1.09 | 0.000361 |
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Target: 5'- gUUGAGGAUUCAGCCCUGUGGGGCGGCg -3' miRNA: 3'- -AACUCCUAAGUCGGGACACCCCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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