Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21751 | 5' | -54.1 | NC_004813.1 | + | 31212 | 0.69 | 0.57172 |
Target: 5'- --uCCGUCaguACCGcaauGGCCGaCACCUUCGCa -3' miRNA: 3'- auuGGCAG---UGGU----UUGGC-GUGGGAGUG- -5' |
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21751 | 5' | -54.1 | NC_004813.1 | + | 42030 | 0.7 | 0.549763 |
Target: 5'- aAACCGccaaaauuagcCACCAuGCCGCGCaUCUCACa -3' miRNA: 3'- aUUGGCa----------GUGGUuUGGCGUG-GGAGUG- -5' |
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21751 | 5' | -54.1 | NC_004813.1 | + | 43848 | 0.7 | 0.538882 |
Target: 5'- --cCCGgcgCGCCAGACCGCugUCaCACc -3' miRNA: 3'- auuGGCa--GUGGUUUGGCGugGGaGUG- -5' |
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21751 | 5' | -54.1 | NC_004813.1 | + | 45061 | 0.7 | 0.505653 |
Target: 5'- --cCUGUCcaucgauGCCGccAGCCGCACCCUgACg -3' miRNA: 3'- auuGGCAG-------UGGU--UUGGCGUGGGAgUG- -5' |
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21751 | 5' | -54.1 | NC_004813.1 | + | 43672 | 0.7 | 0.496168 |
Target: 5'- cAACCGgcgugugCGCCuuGCCGCcACCUUUACc -3' miRNA: 3'- aUUGGCa------GUGGuuUGGCG-UGGGAGUG- -5' |
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21751 | 5' | -54.1 | NC_004813.1 | + | 13561 | 0.71 | 0.455051 |
Target: 5'- -cACCGuUCGCCAGACCuuACCUUCGa -3' miRNA: 3'- auUGGC-AGUGGUUUGGcgUGGGAGUg -5' |
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21751 | 5' | -54.1 | NC_004813.1 | + | 41115 | 1.07 | 0.001621 |
Target: 5'- aUAACCGUCACCAAACCGCACCCUCACc -3' miRNA: 3'- -AUUGGCAGUGGUUUGGCGUGGGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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