miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21752 5' -55.8 NC_004813.1 + 43287 0.66 0.690294
Target:  5'- aGGCACCggCAUCGGGUUCcccuggGCAAucauguuAUCCa -3'
miRNA:   3'- -CCGUGG--GUGGUCCGAGa-----CGUU-------UAGGa -5'
21752 5' -55.8 NC_004813.1 + 38880 0.66 0.669488
Target:  5'- gGGCugCCGCCAcGGC-CUGCuGAgcaCg -3'
miRNA:   3'- -CCGugGGUGGU-CCGaGACGuUUag-Ga -5'
21752 5' -55.8 NC_004813.1 + 44461 0.66 0.669488
Target:  5'- aGGCACCagGCCGGGUuauugCUGUAug-CCg -3'
miRNA:   3'- -CCGUGGg-UGGUCCGa----GACGUuuaGGa -5'
21752 5' -55.8 NC_004813.1 + 46023 0.66 0.655175
Target:  5'- cGCAgauuucCCCACCAGGUugacgCUGCGcacguaaaaccagaAAUCCUu -3'
miRNA:   3'- cCGU------GGGUGGUCCGa----GACGU--------------UUAGGA- -5'
21752 5' -55.8 NC_004813.1 + 32180 0.67 0.614315
Target:  5'- aGGCGCCgGCaaaGGGCUCaGCcaccgCCUg -3'
miRNA:   3'- -CCGUGGgUGg--UCCGAGaCGuuua-GGA- -5'
21752 5' -55.8 NC_004813.1 + 11698 0.68 0.559516
Target:  5'- cGGCGgCCACCGGGgauuuuuuCUUUGUGAuUCCa -3'
miRNA:   3'- -CCGUgGGUGGUCC--------GAGACGUUuAGGa -5'
21752 5' -55.8 NC_004813.1 + 41664 1.09 0.000819
Target:  5'- uGGCACCCACCAGGCUCUGCAAAUCCUc -3'
miRNA:   3'- -CCGUGGGUGGUCCGAGACGUUUAGGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.