miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21753 3' -53.8 NC_004813.1 + 47167 0.66 0.775764
Target:  5'- -uAUCCGGuGCACCUGcAugGGCGu-- -3'
miRNA:   3'- cuUAGGCC-CGUGGACaUugCUGUcgc -5'
21753 3' -53.8 NC_004813.1 + 21334 0.67 0.755252
Target:  5'- --cUCCGGGuCGCUgGUGGCacuGACAGCc -3'
miRNA:   3'- cuuAGGCCC-GUGGaCAUUG---CUGUCGc -5'
21753 3' -53.8 NC_004813.1 + 30352 0.69 0.613621
Target:  5'- --uUCgCGGGCACg-GUGACGGCAGaCa -3'
miRNA:   3'- cuuAG-GCCCGUGgaCAUUGCUGUC-Gc -5'
21753 3' -53.8 NC_004813.1 + 45118 0.7 0.580487
Target:  5'- gGAGaCCGGuGCcgccacggugaACCUGauUAACGGCAGCGg -3'
miRNA:   3'- -CUUaGGCC-CG-----------UGGAC--AUUGCUGUCGC- -5'
21753 3' -53.8 NC_004813.1 + 37793 0.71 0.494603
Target:  5'- aGAUCCagcGGGCGuCCcGUAugGGCAGUGa -3'
miRNA:   3'- cUUAGG---CCCGU-GGaCAUugCUGUCGC- -5'
21753 3' -53.8 NC_004813.1 + 42863 1.08 0.00179
Target:  5'- gGAAUCCGGGCACCUGUAACGACAGCGc -3'
miRNA:   3'- -CUUAGGCCCGUGGACAUUGCUGUCGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.