Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21753 | 5' | -54.4 | NC_004813.1 | + | 42826 | 1.09 | 0.001185 |
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Target: 5'- uUCCGCUGCCGUGUUCACAUACAGGCUg -3' miRNA: 3'- -AGGCGACGGCACAAGUGUAUGUCCGA- -5' |
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| 21753 | 5' | -54.4 | NC_004813.1 | + | 45217 | 0.71 | 0.453444 |
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Target: 5'- aCCGCUGCCGUuaaucagGUUCACcgugGC-GGCa -3' miRNA: 3'- aGGCGACGGCA-------CAAGUGua--UGuCCGa -5' |
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| 21753 | 5' | -54.4 | NC_004813.1 | + | 42925 | 0.69 | 0.570614 |
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Target: 5'- cUCCGgUGCCGUGUcaUCACcgGCAauacguaucuGGUa -3' miRNA: 3'- -AGGCgACGGCACA--AGUGuaUGU----------CCGa -5' |
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| 21753 | 5' | -54.4 | NC_004813.1 | + | 31384 | 0.69 | 0.581628 |
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Target: 5'- aCCGCUugagGCCGUGcccUUCACAUcAUGGGUg -3' miRNA: 3'- aGGCGA----CGGCAC---AAGUGUA-UGUCCGa -5' |
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| 21753 | 5' | -54.4 | NC_004813.1 | + | 30644 | 0.68 | 0.648291 |
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Target: 5'- uUCCGCUGCgGUGUUguCGUAau-GCUc -3' miRNA: 3'- -AGGCGACGgCACAAguGUAUgucCGA- -5' |
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| 21753 | 5' | -54.4 | NC_004813.1 | + | 21559 | 0.67 | 0.670499 |
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Target: 5'- aCUGCUGCCGU--UUAUaccggcgaAUGCGGGCa -3' miRNA: 3'- aGGCGACGGCAcaAGUG--------UAUGUCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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