Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21754 | 3' | -53.9 | NC_004813.1 | + | 40461 | 0.66 | 0.808668 |
Target: 5'- aCCCuccGUcgCAaGCUGuGCugCCuGUGAg -3' miRNA: 3'- -GGGu--CAuaGUaCGAC-CGugGGuCACU- -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 21366 | 0.66 | 0.767958 |
Target: 5'- aCCCGcGUAUUAagcaGCUGGCGCgCCgcaguacGGUGAc -3' miRNA: 3'- -GGGU-CAUAGUa---CGACCGUG-GG-------UCACU- -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 45415 | 0.67 | 0.726994 |
Target: 5'- uUCCGGUA-CGUGCU-GCACCgCcGUGAu -3' miRNA: 3'- -GGGUCAUaGUACGAcCGUGG-GuCACU- -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 45802 | 0.67 | 0.716225 |
Target: 5'- gCCCGGUGUCAgcucaaucgugGCgGGUACCgCAGg-- -3' miRNA: 3'- -GGGUCAUAGUa----------CGaCCGUGG-GUCacu -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 3501 | 0.69 | 0.605749 |
Target: 5'- aCCCGGcUAcCGUGCUGGCACugCUGGaUGAa -3' miRNA: 3'- -GGGUC-AUaGUACGACCGUG--GGUC-ACU- -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 21469 | 0.69 | 0.583606 |
Target: 5'- gCCCGGUGUCAccguacUGC-GGCGCgCCAGcUGc -3' miRNA: 3'- -GGGUCAUAGU------ACGaCCGUG-GGUC-ACu -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 38256 | 0.72 | 0.417222 |
Target: 5'- aCCCAGUucaGUCAggcGCUGGCGCagGGUGu -3' miRNA: 3'- -GGGUCA---UAGUa--CGACCGUGggUCACu -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 41792 | 0.75 | 0.305373 |
Target: 5'- uCCCGGUcgcAUCAUGCUGGCGaacaCCuguauGUGGg -3' miRNA: 3'- -GGGUCA---UAGUACGACCGUg---GGu----CACU- -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 5292 | 0.76 | 0.248129 |
Target: 5'- gUCCGGUGUCAUGCUGcCACCuucugcuCAGUGGc -3' miRNA: 3'- -GGGUCAUAGUACGACcGUGG-------GUCACU- -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 49689 | 0.8 | 0.148706 |
Target: 5'- cCCCGGUAucccgcUCAUGCUGGCgaACCCAGa-- -3' miRNA: 3'- -GGGUCAU------AGUACGACCG--UGGGUCacu -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 52526 | 0.8 | 0.148706 |
Target: 5'- cCCCGGUAucccgcUCAUGCUGGCgaACCCAGa-- -3' miRNA: 3'- -GGGUCAU------AGUACGACCG--UGGGUCacu -5' |
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21754 | 3' | -53.9 | NC_004813.1 | + | 43158 | 1.12 | 0.000885 |
Target: 5'- aCCCAGUAUCAUGCUGGCACCCAGUGAa -3' miRNA: 3'- -GGGUCAUAGUACGACCGUGGGUCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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