Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21754 | 5' | -60.6 | NC_004813.1 | + | 12860 | 0.66 | 0.442677 |
Target: 5'- aGACuCGCCGGgaaagUUGCCAGuaacuGGCUGAACacaCCg -3' miRNA: 3'- -UUG-GUGGCC-----GACGGUC-----CCGACUUG---GG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 34306 | 0.66 | 0.433229 |
Target: 5'- uGCCcuGCCGGCU-CCGcaggacGGGCgUGAugCCa -3' miRNA: 3'- uUGG--UGGCCGAcGGU------CCCG-ACUugGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 38885 | 0.66 | 0.418359 |
Target: 5'- -cCCGCgGGCUGCCGccacGGccuGCUGAgcacgguacacggcuGCCCu -3' miRNA: 3'- uuGGUGgCCGACGGU----CC---CGACU---------------UGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 32162 | 0.66 | 0.41469 |
Target: 5'- cAGCCACCGcCUGaaaCA-GGCUGGuccaGCCCg -3' miRNA: 3'- -UUGGUGGCcGACg--GUcCCGACU----UGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 41065 | 0.66 | 0.41469 |
Target: 5'- cGCCGCCcca---CAGGGCUGAAUCCu -3' miRNA: 3'- uUGGUGGccgacgGUCCCGACUUGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 34848 | 0.66 | 0.405605 |
Target: 5'- uACCGCCGuGCUGCuggccuacauuCAGGGC--AGCCg -3' miRNA: 3'- uUGGUGGC-CGACG-----------GUCCCGacUUGGg -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 48607 | 0.66 | 0.387817 |
Target: 5'- --aCACCGGCccUGUCGGGGC--AACCg -3' miRNA: 3'- uugGUGGCCG--ACGGUCCCGacUUGGg -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 24324 | 0.66 | 0.387817 |
Target: 5'- cGGCCugUcGGCUGCCGuucuGGcGCUGAuuGCCg -3' miRNA: 3'- -UUGGugG-CCGACGGU----CC-CGACU--UGGg -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 30957 | 0.67 | 0.370548 |
Target: 5'- aGACCAUUaucgGGCgauuugGUCAGGGCgGGAUCCc -3' miRNA: 3'- -UUGGUGG----CCGa-----CGGUCCCGaCUUGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 42320 | 0.67 | 0.352169 |
Target: 5'- cAGCCGCCGGUcagccucgcucaGCCAGGGCaGuccACCg -3' miRNA: 3'- -UUGGUGGCCGa-----------CGGUCCCGaCu--UGGg -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 48328 | 0.67 | 0.345648 |
Target: 5'- -uCUGCCGcCUGCC-GGGCUGAcUCCg -3' miRNA: 3'- uuGGUGGCcGACGGuCCCGACUuGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 21704 | 0.68 | 0.329726 |
Target: 5'- uGCCAUCGGCU-CCGGuggugaacGCUGAACCa -3' miRNA: 3'- uUGGUGGCCGAcGGUCc-------CGACUUGGg -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 21218 | 0.68 | 0.292319 |
Target: 5'- uGGCCACCGGaCUaCCAaacGGCUGccAGCCCu -3' miRNA: 3'- -UUGGUGGCC-GAcGGUc--CCGAC--UUGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 27654 | 0.69 | 0.276257 |
Target: 5'- uGCCGCCGGUUauucggcgaagacaGCCAGGGUUaaaGGCUCg -3' miRNA: 3'- uUGGUGGCCGA--------------CGGUCCCGAc--UUGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 38627 | 0.69 | 0.271511 |
Target: 5'- gGGCgCGCUGGUUGCCgucGGGGUU--GCCCg -3' miRNA: 3'- -UUG-GUGGCCGACGG---UCCCGAcuUGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 48692 | 0.69 | 0.271511 |
Target: 5'- -uUCGCCGGUUGCCccgacAGGGCcGGugUCa -3' miRNA: 3'- uuGGUGGCCGACGG-----UCCCGaCUugGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 38340 | 0.69 | 0.264845 |
Target: 5'- cGugCACUGGCUGCgGguauGGGCguGGCCCg -3' miRNA: 3'- -UugGUGGCCGACGgU----CCCGacUUGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 30127 | 0.7 | 0.245641 |
Target: 5'- cGCaACUGGCUGCCA-GGCUGGaugagGCCUg -3' miRNA: 3'- uUGgUGGCCGACGGUcCCGACU-----UGGG- -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 32583 | 0.76 | 0.093899 |
Target: 5'- uGCCGCCGGUguaUGCCGccGGGCUGGcgGCCa -3' miRNA: 3'- uUGGUGGCCG---ACGGU--CCCGACU--UGGg -5' |
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21754 | 5' | -60.6 | NC_004813.1 | + | 43109 | 1.08 | 0.000333 |
Target: 5'- aAACCACCGGCUGCCAGGGCUGAACCCc -3' miRNA: 3'- -UUGGUGGCCGACGGUCCCGACUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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