Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21757 | 5' | -58.1 | NC_004813.1 | + | 1354 | 0.66 | 0.512189 |
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Target: 5'- cAGACGUCCAggagcauuaaCAGGUuaACCGGAa- -3' miRNA: 3'- -UCUGCGGGUac--------GUCCAcgUGGCCUau -5' |
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| 21757 | 5' | -58.1 | NC_004813.1 | + | 42776 | 0.66 | 0.512189 |
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Target: 5'- cGGGCGCUguCGUuaCAGGUGC-CCGGAUu -3' miRNA: 3'- -UCUGCGG--GUAc-GUCCACGuGGCCUAu -5' |
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| 21757 | 5' | -58.1 | NC_004813.1 | + | 53121 | 0.67 | 0.481369 |
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Target: 5'- gAGACGacauUCGUGCuGGaUGCACUGGAg- -3' miRNA: 3'- -UCUGCg---GGUACGuCC-ACGUGGCCUau -5' |
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| 21757 | 5' | -58.1 | NC_004813.1 | + | 44904 | 0.67 | 0.441741 |
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Target: 5'- cAGGCcCCCGUGCcGG-GCugUGGGUGa -3' miRNA: 3'- -UCUGcGGGUACGuCCaCGugGCCUAU- -5' |
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| 21757 | 5' | -58.1 | NC_004813.1 | + | 22378 | 0.69 | 0.385948 |
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Target: 5'- uGACGCCUGUgGCuucUGCACCGGAa- -3' miRNA: 3'- uCUGCGGGUA-CGuccACGUGGCCUau -5' |
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| 21757 | 5' | -58.1 | NC_004813.1 | + | 35369 | 0.7 | 0.327015 |
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Target: 5'- cGGGCuGCCCGUGCcGGaUGCgACCGGcgGg -3' miRNA: 3'- -UCUG-CGGGUACGuCC-ACG-UGGCCuaU- -5' |
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| 21757 | 5' | -58.1 | NC_004813.1 | + | 30038 | 0.7 | 0.31916 |
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Target: 5'- --cCGCCCuguggggcaGUGUAcGGUGCGCCGGGUc -3' miRNA: 3'- ucuGCGGG---------UACGU-CCACGUGGCCUAu -5' |
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| 21757 | 5' | -58.1 | NC_004813.1 | + | 47250 | 1.06 | 0.000746 |
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Target: 5'- cAGACGCCCAUGCAGGUGCACCGGAUAc -3' miRNA: 3'- -UCUGCGGGUACGUCCACGUGGCCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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