Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21758 | 3' | -53.9 | NC_004813.1 | + | 31285 | 0.66 | 0.797393 |
Target: 5'- ---cGCCACcaccgcuUCCGCCAgCGCAuUACGg -3' miRNA: 3'- cauuCGGUGu------GGGCGGUaGCGU-AUGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 11314 | 0.66 | 0.787514 |
Target: 5'- -aAGGCCACAUgCGuuuCCA-CGCAUACc -3' miRNA: 3'- caUUCGGUGUGgGC---GGUaGCGUAUGc -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 33578 | 0.66 | 0.787514 |
Target: 5'- gGUAAGCCAUAUCC-CUGUucCGCGUgACGg -3' miRNA: 3'- -CAUUCGGUGUGGGcGGUA--GCGUA-UGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 39006 | 0.66 | 0.787514 |
Target: 5'- cGUGAGCC-CA-CCGCCGUUGUAc--- -3' miRNA: 3'- -CAUUCGGuGUgGGCGGUAGCGUaugc -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 41558 | 0.66 | 0.777469 |
Target: 5'- -aAAGCCACugCC-CUcaAUCGgGUACGc -3' miRNA: 3'- caUUCGGUGugGGcGG--UAGCgUAUGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 35460 | 0.66 | 0.777469 |
Target: 5'- ------gACGCCCGCCGgUCGCAUcCGg -3' miRNA: 3'- cauucggUGUGGGCGGU-AGCGUAuGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 50211 | 0.66 | 0.771368 |
Target: 5'- ---uGCCACGaacugacgguuuaCGCgGUCGCAUGCGg -3' miRNA: 3'- cauuCGGUGUgg-----------GCGgUAGCGUAUGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 31632 | 0.66 | 0.75693 |
Target: 5'- cUGAGCCGaccggcgacagUACCaCGCCGUCGCcgGUGg -3' miRNA: 3'- cAUUCGGU-----------GUGG-GCGGUAGCGuaUGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 42120 | 0.68 | 0.659408 |
Target: 5'- --cAGgCGCACaCCGCCGg-GCAUGCGc -3' miRNA: 3'- cauUCgGUGUG-GGCGGUagCGUAUGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 12640 | 0.69 | 0.637159 |
Target: 5'- ---uGUCACGCCUGCCuGUUGCuuguUACGg -3' miRNA: 3'- cauuCGGUGUGGGCGG-UAGCGu---AUGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 44969 | 0.69 | 0.626023 |
Target: 5'- gGUGGGCCugACCCGaCUggUGCAgcCGa -3' miRNA: 3'- -CAUUCGGugUGGGC-GGuaGCGUauGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 18618 | 0.69 | 0.602665 |
Target: 5'- -cGAGCCauACACCCGCauauaauCAUCGCGUcauCGu -3' miRNA: 3'- caUUCGG--UGUGGGCG-------GUAGCGUAu--GC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 22275 | 0.71 | 0.50588 |
Target: 5'- -aGAGgUGCGCCCGUCAUCGCcuuccgGUGCa -3' miRNA: 3'- caUUCgGUGUGGGCGGUAGCG------UAUGc -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 38428 | 0.71 | 0.495383 |
Target: 5'- -cGGGCCACGCCCauacccgcaGCCAgUGCAcgUACGa -3' miRNA: 3'- caUUCGGUGUGGG---------CGGUaGCGU--AUGC- -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 22568 | 0.72 | 0.424975 |
Target: 5'- -aGAGCCGCACCguUGCCcgCgGCGUACu -3' miRNA: 3'- caUUCGGUGUGG--GCGGuaG-CGUAUGc -5' |
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21758 | 3' | -53.9 | NC_004813.1 | + | 47538 | 1.08 | 0.001653 |
Target: 5'- uGUAAGCCACACCCGCCAUCGCAUACGc -3' miRNA: 3'- -CAUUCGGUGUGGGCGGUAGCGUAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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