Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21758 | 5' | -57.4 | NC_004813.1 | + | 28295 | 0.66 | 0.616363 |
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Target: 5'- -aCGUUCGgggaguccUGCGCACAGugGGUguuugaCCUGg -3' miRNA: 3'- aaGUAAGU--------GCGCGUGUCugCCG------GGGC- -5' |
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| 21758 | 5' | -57.4 | NC_004813.1 | + | 41010 | 0.66 | 0.605473 |
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Target: 5'- cUCcgUCACGuCGCAUGGACaGCaucaCCGc -3' miRNA: 3'- aAGuaAGUGC-GCGUGUCUGcCGg---GGC- -5' |
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| 21758 | 5' | -57.4 | NC_004813.1 | + | 44487 | 0.67 | 0.530334 |
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Target: 5'- aUCGggCAguacUGCGaC-CAGACGGUCCCGg -3' miRNA: 3'- aAGUaaGU----GCGC-GuGUCUGCCGGGGC- -5' |
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| 21758 | 5' | -57.4 | NC_004813.1 | + | 39129 | 0.67 | 0.509445 |
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Target: 5'- -gCAUaCugGCGCGCAGACu-CCCUGg -3' miRNA: 3'- aaGUAaGugCGCGUGUCUGccGGGGC- -5' |
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| 21758 | 5' | -57.4 | NC_004813.1 | + | 30355 | 0.69 | 0.401897 |
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Target: 5'- -gCAUUCGCGgGCACGguGACGGCagacaCCu -3' miRNA: 3'- aaGUAAGUGCgCGUGU--CUGCCGg----GGc -5' |
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| 21758 | 5' | -57.4 | NC_004813.1 | + | 29362 | 0.72 | 0.260223 |
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Target: 5'- ---uUUCAUGCGCcCAGGCauGGCCCCa -3' miRNA: 3'- aaguAAGUGCGCGuGUCUG--CCGGGGc -5' |
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| 21758 | 5' | -57.4 | NC_004813.1 | + | 47502 | 1.06 | 0.001045 |
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Target: 5'- aUUCAUUCACGCGCACAGACGGCCCCGc -3' miRNA: 3'- -AAGUAAGUGCGCGUGUCUGCCGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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