miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21759 3' -51.5 NC_004813.1 + 47761 1.08 0.003191
Target:  5'- cCGACACCCACGAUGAAACCGUCAGUGc -3'
miRNA:   3'- -GCUGUGGGUGCUACUUUGGCAGUCAC- -5'
21759 3' -51.5 NC_004813.1 + 30568 0.68 0.819871
Target:  5'- aCGACAacaCCGCaGcgGAAAuCCGUCAgGUGg -3'
miRNA:   3'- -GCUGUg--GGUG-CuaCUUU-GGCAGU-CAC- -5'
21759 3' -51.5 NC_004813.1 + 29283 0.68 0.847034
Target:  5'- gGGCcauGCCUggGCGcAUGAAACCG-CGGUGg -3'
miRNA:   3'- gCUG---UGGG--UGC-UACUUUGGCaGUCAC- -5'
21759 3' -51.5 NC_004813.1 + 15259 0.67 0.863998
Target:  5'- --cCACCCACGGccUGAuauuGCUGUaCGGUGg -3'
miRNA:   3'- gcuGUGGGUGCU--ACUu---UGGCA-GUCAC- -5'
21759 3' -51.5 NC_004813.1 + 52961 0.67 0.88756
Target:  5'- aGGCGaCCGCGu--AAACCGUCAGUu -3'
miRNA:   3'- gCUGUgGGUGCuacUUUGGCAGUCAc -5'
21759 3' -51.5 NC_004813.1 + 21384 0.66 0.901937
Target:  5'- uGGCGCgCCgcaguACGGUGAcACCGggCGGUGc -3'
miRNA:   3'- gCUGUG-GG-----UGCUACUuUGGCa-GUCAC- -5'
21759 3' -51.5 NC_004813.1 + 35939 0.66 0.908712
Target:  5'- uGugGuCCcccugCugGAUGGcaGGCCGUCGGUGg -3'
miRNA:   3'- gCugU-GG-----GugCUACU--UUGGCAGUCAC- -5'
21759 3' -51.5 NC_004813.1 + 36107 0.66 0.913287
Target:  5'- gGACGCCCGUGAagccauaaugcugcUGGccccgggauCCGUCAGUGa -3'
miRNA:   3'- gCUGUGGGUGCU--------------ACUuu-------GGCAGUCAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.