Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21759 | 5' | -54.2 | NC_004813.1 | + | 28815 | 0.66 | 0.751064 |
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Target: 5'- aGCCGCcaGCCGGGGUgUUCAgacagaaacuGCAGu -3' miRNA: 3'- -CGGUGa-CGGCCUCGgAAGUauu-------CGUU- -5' |
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| 21759 | 5' | -54.2 | NC_004813.1 | + | 25037 | 0.66 | 0.73173 |
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Target: 5'- uUgGCUGUCGGuGCCUUUcuGUGGGCu- -3' miRNA: 3'- cGgUGACGGCCuCGGAAG--UAUUCGuu -5' |
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| 21759 | 5' | -54.2 | NC_004813.1 | + | 56171 | 0.66 | 0.70985 |
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Target: 5'- uGCCAUUGauugugguggaCUGGAGCCUUCu---GCAc -3' miRNA: 3'- -CGGUGAC-----------GGCCUCGGAAGuauuCGUu -5' |
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| 21759 | 5' | -54.2 | NC_004813.1 | + | 42923 | 0.66 | 0.698784 |
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Target: 5'- uGCCGCgacugGCCGGGGCCggaaaGGGUGGa -3' miRNA: 3'- -CGGUGa----CGGCCUCGGaaguaUUCGUU- -5' |
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| 21759 | 5' | -54.2 | NC_004813.1 | + | 27236 | 0.67 | 0.642667 |
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Target: 5'- -aCGCUGCCGGAGguguCCggaggUCAUGgcagGGCAGa -3' miRNA: 3'- cgGUGACGGCCUC----GGa----AGUAU----UCGUU- -5' |
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| 21759 | 5' | -54.2 | NC_004813.1 | + | 42997 | 0.69 | 0.530888 |
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Target: 5'- uUCACUGCCGGGGCa-UCGauGGCGu -3' miRNA: 3'- cGGUGACGGCCUCGgaAGUauUCGUu -5' |
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| 21759 | 5' | -54.2 | NC_004813.1 | + | 37095 | 0.71 | 0.407587 |
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Target: 5'- --uGCUGCgGGAGCCUUCGgcagAGGCc- -3' miRNA: 3'- cggUGACGgCCUCGGAAGUa---UUCGuu -5' |
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| 21759 | 5' | -54.2 | NC_004813.1 | + | 49782 | 0.76 | 0.215658 |
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Target: 5'- uGCCACUGCUGGAuaaGCUg-CGUGAGCAGu -3' miRNA: 3'- -CGGUGACGGCCU---CGGaaGUAUUCGUU- -5' |
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| 21759 | 5' | -54.2 | NC_004813.1 | + | 52619 | 0.76 | 0.215658 |
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Target: 5'- uGCCACUGCUGGAuaaGCUg-CGUGAGCAGu -3' miRNA: 3'- -CGGUGACGGCCU---CGGaaGUAUUCGUU- -5' |
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| 21759 | 5' | -54.2 | NC_004813.1 | + | 47727 | 1.08 | 0.001124 |
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Target: 5'- cGCCACUGCCGGAGCCUUCAUAAGCAAu -3' miRNA: 3'- -CGGUGACGGCCUCGGAAGUAUUCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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