miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21760 5' -53.9 NC_004813.1 + 34309 0.66 0.768952
Target:  5'- cCUGcCGGC-UCCGCaggacggGCGUgaugCCACUGCAc -3'
miRNA:   3'- -GAC-GUUGuAGGCGa------CGUA----GGUGACGU- -5'
21760 5' -53.9 NC_004813.1 + 54858 0.66 0.768952
Target:  5'- aCUGCAGUGUCgacuaUUGCAUCUGCUGCAa -3'
miRNA:   3'- -GACGUUGUAGgc---GACGUAGGUGACGU- -5'
21760 5' -53.9 NC_004813.1 + 31431 0.66 0.76479
Target:  5'- gCUGCAACAaacuguccaaacgCCGCcucUGCAUCCGCa--- -3'
miRNA:   3'- -GACGUUGUa------------GGCG---ACGUAGGUGacgu -5'
21760 5' -53.9 NC_004813.1 + 40135 0.66 0.758504
Target:  5'- uUGCAGCAgaUCCGCgacaGCuUCCGCcagaaggaUGCGg -3'
miRNA:   3'- gACGUUGU--AGGCGa---CGuAGGUG--------ACGU- -5'
21760 5' -53.9 NC_004813.1 + 35319 0.66 0.757452
Target:  5'- cCUGCGcCAuuUCCGCaccggacUGCAUCCGCUcccgGCu -3'
miRNA:   3'- -GACGUuGU--AGGCG-------ACGUAGGUGA----CGu -5'
21760 5' -53.9 NC_004813.1 + 38898 0.66 0.737214
Target:  5'- uCUGCAucucCGUCCcgcggGCUGCcgCCACgGCc -3'
miRNA:   3'- -GACGUu---GUAGG-----CGACGuaGGUGaCGu -5'
21760 5' -53.9 NC_004813.1 + 32306 0.67 0.71548
Target:  5'- cCUGC-GCAUCCGuCUGCAUaagaCGCaGCc -3'
miRNA:   3'- -GACGuUGUAGGC-GACGUAg---GUGaCGu -5'
21760 5' -53.9 NC_004813.1 + 46294 0.67 0.686724
Target:  5'- gUGCAGCAaggucaucauuugugUCCuucugcacgcGCUGUAUCUGCUGUAu -3'
miRNA:   3'- gACGUUGU---------------AGG----------CGACGUAGGUGACGU- -5'
21760 5' -53.9 NC_004813.1 + 2129 0.7 0.525975
Target:  5'- cCUGCGGCGguUCUgguaGCUGCAUCCAgUGgGu -3'
miRNA:   3'- -GACGUUGU--AGG----CGACGUAGGUgACgU- -5'
21760 5' -53.9 NC_004813.1 + 48496 0.71 0.452476
Target:  5'- uCUGUuGCggUCGUUGCAUCCcugGCUGCAu -3'
miRNA:   3'- -GACGuUGuaGGCGACGUAGG---UGACGU- -5'
21760 5' -53.9 NC_004813.1 + 54822 1.09 0.001159
Target:  5'- gCUGCAACAUCCGCUGCAUCCACUGCAg -3'
miRNA:   3'- -GACGUUGUAGGCGACGUAGGUGACGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.