Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21762 | 5' | -55.4 | NC_004813.1 | + | 48598 | 0.66 | 0.723767 |
Target: 5'- aGCGUGGUGacaCCGGcccuGucGggGCAaCCGg -3' miRNA: 3'- aCGUACCAC---GGCC----CuuCuuCGUaGGC- -5' |
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21762 | 5' | -55.4 | NC_004813.1 | + | 35214 | 0.66 | 0.669488 |
Target: 5'- gGCA-GGagcaGCCGGGAgcGGAuGCAgUCCGg -3' miRNA: 3'- aCGUaCCa---CGGCCCU--UCUuCGU-AGGC- -5' |
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21762 | 5' | -55.4 | NC_004813.1 | + | 29028 | 0.67 | 0.625358 |
Target: 5'- aGCccGGgagGCUGGuGAGGAAGCAUUUc -3' miRNA: 3'- aCGuaCCa--CGGCC-CUUCUUCGUAGGc -5' |
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21762 | 5' | -55.4 | NC_004813.1 | + | 31874 | 0.67 | 0.614315 |
Target: 5'- cGCAUGaUGcCCGGGccGggGaUAUCCGu -3' miRNA: 3'- aCGUACcAC-GGCCCuuCuuC-GUAGGC- -5' |
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21762 | 5' | -55.4 | NC_004813.1 | + | 27557 | 0.68 | 0.581317 |
Target: 5'- gUGCGuucUGGacCCGGGAGGAGGCugugCUGa -3' miRNA: 3'- -ACGU---ACCacGGCCCUUCUUCGua--GGC- -5' |
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21762 | 5' | -55.4 | NC_004813.1 | + | 18756 | 0.7 | 0.435475 |
Target: 5'- aGCGUGaauUGCCGGGAGGGAGaaccUCUGu -3' miRNA: 3'- aCGUACc--ACGGCCCUUCUUCgu--AGGC- -5' |
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21762 | 5' | -55.4 | NC_004813.1 | + | 37088 | 0.71 | 0.397727 |
Target: 5'- aGCGUGGUGCUGcGGGAGccuucGGCAgaggCCu -3' miRNA: 3'- aCGUACCACGGC-CCUUCu----UCGUa---GGc -5' |
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21762 | 5' | -55.4 | NC_004813.1 | + | 12959 | 1 | 0.004141 |
Target: 5'- uUGCAUGGUGCCGGG-AGAAGCAUCCGu -3' miRNA: 3'- -ACGUACCACGGCCCuUCUUCGUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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