Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21763 | 3' | -51.7 | NC_004813.1 | + | 50694 | 0.66 | 0.889261 |
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Target: 5'- aGCAUAAUaAGCUuuuUCUGcuucUGCCGGAg -3' miRNA: 3'- gCGUAUUA-UCGA---AGACac--GCGGCCUg -5' |
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| 21763 | 3' | -51.7 | NC_004813.1 | + | 53531 | 0.66 | 0.889261 |
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Target: 5'- aGCAUAAUaAGCUuuuUCUGcuucUGCCGGAg -3' miRNA: 3'- gCGUAUUA-UCGA---AGACac--GCGGCCUg -5' |
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| 21763 | 3' | -51.7 | NC_004813.1 | + | 25865 | 0.66 | 0.856975 |
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Target: 5'- gGCGgu-UGGCUgugGUGCGCUGuGGCu -3' miRNA: 3'- gCGUauuAUCGAagaCACGCGGC-CUG- -5' |
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| 21763 | 3' | -51.7 | NC_004813.1 | + | 22376 | 0.66 | 0.856975 |
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Target: 5'- aCGCcu-GUGGCUUCUGcaccggaagGCGaugaCGGGCg -3' miRNA: 3'- -GCGuauUAUCGAAGACa--------CGCg---GCCUG- -5' |
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| 21763 | 3' | -51.7 | NC_004813.1 | + | 17489 | 0.66 | 0.856975 |
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Target: 5'- uGCAU--UGGCacau-UGCGCCGGGCa -3' miRNA: 3'- gCGUAuuAUCGaagacACGCGGCCUG- -5' |
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| 21763 | 3' | -51.7 | NC_004813.1 | + | 14707 | 0.67 | 0.811063 |
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Target: 5'- gGCAUGGUcaacGGCUg----GCGCCGGAa -3' miRNA: 3'- gCGUAUUA----UCGAagacaCGCGGCCUg -5' |
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| 21763 | 3' | -51.7 | NC_004813.1 | + | 43765 | 0.69 | 0.727849 |
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Target: 5'- gGUGUGAcAGCggUCUGgcGCGCCGGGg -3' miRNA: 3'- gCGUAUUaUCGa-AGACa-CGCGGCCUg -5' |
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| 21763 | 3' | -51.7 | NC_004813.1 | + | 47409 | 0.71 | 0.592703 |
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Target: 5'- aGCGUAAUGGCggggccgUCUGUGCGCg---- -3' miRNA: 3'- gCGUAUUAUCGa------AGACACGCGgccug -5' |
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| 21763 | 3' | -51.7 | NC_004813.1 | + | 13373 | 1.12 | 0.001407 |
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Target: 5'- aCGCAUAAUAGCUUCUGUGCGCCGGACg -3' miRNA: 3'- -GCGUAUUAUCGAAGACACGCGGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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