Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21764 | 5' | -51.3 | NC_004813.1 | + | 45435 | 0.66 | 0.868195 |
Target: 5'- uGCAGCAccugacgguggagguGAGCGCAGcuGAcGGCCaGUAUc -3' miRNA: 3'- gCGUCGU---------------UUCGCGUC--UUaCUGG-CAUA- -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 12299 | 0.66 | 0.864841 |
Target: 5'- gGCAGCAAguggGGaacaGCAGAAgaccUGACCGc-- -3' miRNA: 3'- gCGUCGUU----UCg---CGUCUU----ACUGGCaua -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 52734 | 0.66 | 0.864841 |
Target: 5'- aCGCAGUgcccguGCGCAGcgcGAUGACUGg-- -3' miRNA: 3'- -GCGUCGuuu---CGCGUC---UUACUGGCaua -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 16974 | 0.67 | 0.847447 |
Target: 5'- aCGUcuGGCAAAGCGUGGggUGAUUc--- -3' miRNA: 3'- -GCG--UCGUUUCGCGUCuuACUGGcaua -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 43591 | 0.67 | 0.829071 |
Target: 5'- gGCGGCAAGGCGCAcac--GCCGg-- -3' miRNA: 3'- gCGUCGUUUCGCGUcuuacUGGCaua -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 24153 | 0.68 | 0.809795 |
Target: 5'- aGCGGCccaucauuuuaGAGGCGCAGGAUGugUu--- -3' miRNA: 3'- gCGUCG-----------UUUCGCGUCUUACugGcaua -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 24417 | 0.68 | 0.787659 |
Target: 5'- uGCGGCAAucAGCGCcAGAAcggcagccgacaGGCCGUAUu -3' miRNA: 3'- gCGUCGUU--UCGCG-UCUUa-----------CUGGCAUA- -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 39549 | 0.68 | 0.768909 |
Target: 5'- aGCGGCAGGGacuGguGAAUGuGCCGUu- -3' miRNA: 3'- gCGUCGUUUCg--CguCUUAC-UGGCAua -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 37996 | 0.69 | 0.758272 |
Target: 5'- cCGaCAGcCGAAGCGCGGAuuu-CCGUAa -3' miRNA: 3'- -GC-GUC-GUUUCGCGUCUuacuGGCAUa -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 48483 | 0.69 | 0.747496 |
Target: 5'- aGCAGgAuAGCgGCGGAAgaGGCCGUAa -3' miRNA: 3'- gCGUCgUuUCG-CGUCUUa-CUGGCAUa -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 33631 | 0.7 | 0.669224 |
Target: 5'- uGCAG-AAGGCGguGAAUGACgCGg-- -3' miRNA: 3'- gCGUCgUUUCGCguCUUACUG-GCaua -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 33793 | 0.7 | 0.669224 |
Target: 5'- uGCAG-AAGGCGguGAAUGACgCGg-- -3' miRNA: 3'- gCGUCgUUUCGCguCUUACUG-GCaua -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 49117 | 0.72 | 0.531613 |
Target: 5'- uGCAGCGucuGUGCGGuugcggaaagcauGAUGACCGUGa -3' miRNA: 3'- gCGUCGUuu-CGCGUC-------------UUACUGGCAUa -5' |
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21764 | 5' | -51.3 | NC_004813.1 | + | 14642 | 1.07 | 0.003037 |
Target: 5'- gCGCAGCAAAGCGCAGAAUGACCGUAUg -3' miRNA: 3'- -GCGUCGUUUCGCGUCUUACUGGCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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