miRNA: 3'- aGUCUccACGCGGGCAGUAGCGGaag -5'
miRNA: 3'- -AGUcucCACGCGGGCAGuAgCGGAAG- -5'
miRNA: 3'- -AGUCUCCACGCGGGCAGUAGCGGAAG- -5'
miRNA: 3'- -AGUCUCC-ACGC-GG-GCAGuAGCgGAAG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21766 | 3' | -57.3 | NC_004813.1 | + | 42733 | 0.67 | 0.559252 |
|
Target: 5'- aCAGAa-UGCGUUCGUCGUUGCCa-- -3' miRNA: 3'- aGUCUccACGCGGGCAGUAGCGGaag -5' |
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| 21766 | 3' | -57.3 | NC_004813.1 | + | 5673 | 0.69 | 0.427543 |
|
Target: 5'- uUCA---GUGUGCCCGUC-UgGCCUUCg -3' miRNA: 3'- -AGUcucCACGCGGGCAGuAgCGGAAG- -5' |
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| 21766 | 3' | -57.3 | NC_004813.1 | + | 22272 | 1.09 | 0.000639 |
|
Target: 5'- aUCAGAGGUGCGCCCGUCAUCGCCUUCc -3' miRNA: 3'- -AGUCUCCACGCGGGCAGUAGCGGAAG- -5' |
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| 21766 | 3' | -57.3 | NC_004813.1 | + | 48293 | 0.73 | 0.23383 |
|
Target: 5'- gUCAGAGGaUGCGuCCuCGUCcUCGgCUUCu -3' miRNA: 3'- -AGUCUCC-ACGC-GG-GCAGuAGCgGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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