Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21768 | 3' | -54.9 | NC_004813.1 | + | 27713 | 0.66 | 0.747545 |
Target: 5'- cGGAGGUGCUugCGUuuauagcccguaaacAGugcGAGaCGGUGGa -3' miRNA: 3'- -CCUCUAUGGugGCG---------------UCu--CUC-GCCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 33622 | 0.67 | 0.711737 |
Target: 5'- cGGA---AgUACUGCAGAaGGCGGUGAa -3' miRNA: 3'- -CCUcuaUgGUGGCGUCUcUCGCCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 45261 | 0.67 | 0.690226 |
Target: 5'- --cGGU-CCGgCGCGGGGuguGCGGUGAu -3' miRNA: 3'- ccuCUAuGGUgGCGUCUCu--CGCCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 48647 | 0.67 | 0.683726 |
Target: 5'- aGGAGAUACagguccggcagguCCGCAG-GGGCcgaaaGGUGAc -3' miRNA: 3'- -CCUCUAUGgu-----------GGCGUCuCUCG-----CCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 33149 | 0.68 | 0.657571 |
Target: 5'- gGGAGA-ACCuGCCGCcccuGGccGGCGGUGAu -3' miRNA: 3'- -CCUCUaUGG-UGGCG----UCucUCGCCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 31742 | 0.68 | 0.624704 |
Target: 5'- ---cGUACCGCgGCGGuG-GCGGUGAu -3' miRNA: 3'- ccucUAUGGUGgCGUCuCuCGCCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 38830 | 0.68 | 0.613748 |
Target: 5'- cGGAGAUGCagauuGCUGCAGAGgcccgucuGGCGGc-- -3' miRNA: 3'- -CCUCUAUGg----UGGCGUCUC--------UCGCCacu -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 12308 | 0.69 | 0.582098 |
Target: 5'- uGGGGAacagcagaagaccugACCGCCGCAGAGuGgauguuugacaUGGUGAa -3' miRNA: 3'- -CCUCUa--------------UGGUGGCGUCUCuC-----------GCCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 27948 | 0.71 | 0.465689 |
Target: 5'- aGGAGAUAUCACCac---GGGCGGUGGc -3' miRNA: 3'- -CCUCUAUGGUGGcgucuCUCGCCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 34575 | 0.73 | 0.371156 |
Target: 5'- cGGuGAUGCUcuACCGCGGuguguucAGAGCcGGUGAa -3' miRNA: 3'- -CCuCUAUGG--UGGCGUC-------UCUCG-CCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 27860 | 0.78 | 0.173286 |
Target: 5'- cGGAGAUGCCACCGCcc---GUGGUGAu -3' miRNA: 3'- -CCUCUAUGGUGGCGucucuCGCCACU- -5' |
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21768 | 3' | -54.9 | NC_004813.1 | + | 23520 | 1.11 | 0.000864 |
Target: 5'- gGGAGAUACCACCGCAGAGAGCGGUGAa -3' miRNA: 3'- -CCUCUAUGGUGGCGUCUCUCGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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