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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 21769 | 5' | -57.8 | NC_004813.1 | + | 30127 | 0.66 | 0.525462 |
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Target: 5'- ----cGCAACUGGCUgCCaGGCUGGa- -3' miRNA: 3'- uauuaCGUUGGCCGGgGG-CUGACCgu -5' |
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| 21769 | 5' | -57.8 | NC_004813.1 | + | 28090 | 0.67 | 0.463822 |
|
Target: 5'- -cGGUGCcACCaccaCCCCGACUGGUg -3' miRNA: 3'- uaUUACGuUGGccg-GGGGCUGACCGu -5' |
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| 21769 | 5' | -57.8 | NC_004813.1 | + | 34303 | 0.67 | 0.504533 |
|
Target: 5'- -gAGUGCccuGCCGGCUCCgcagGACgGGCGu -3' miRNA: 3'- uaUUACGu--UGGCCGGGGg---CUGaCCGU- -5' |
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| 21769 | 5' | -57.8 | NC_004813.1 | + | 26733 | 0.68 | 0.434446 |
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Target: 5'- ---cUGCuGCCaGCUCCCGGC-GGCAa -3' miRNA: 3'- uauuACGuUGGcCGGGGGCUGaCCGU- -5' |
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| 21769 | 5' | -57.8 | NC_004813.1 | + | 45745 | 0.68 | 0.444119 |
|
Target: 5'- ----gGUGGCCGGuuCCCCCuGCUGGCc -3' miRNA: 3'- uauuaCGUUGGCC--GGGGGcUGACCGu -5' |
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| 21769 | 5' | -57.8 | NC_004813.1 | + | 23335 | 0.7 | 0.34491 |
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Target: 5'- ---uUGCuuCCGGCUgaCUGACUGGCGc -3' miRNA: 3'- uauuACGuuGGCCGGg-GGCUGACCGU- -5' |
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| 21769 | 5' | -57.8 | NC_004813.1 | + | 26165 | 1.07 | 0.000715 |
|
Target: 5'- gAUAAUGCAACCGGCCCCCGACUGGCAg -3' miRNA: 3'- -UAUUACGUUGGCCGGGGGCUGACCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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