miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21770 3' -60.7 NC_004813.1 + 36101 0.66 0.506271
Target:  5'- aCGUCaGGACgcccGUGaagccauaaugcuGCUgGCCCCGGGa -3'
miRNA:   3'- aGUAG-CCUGa---CAC-------------UGGgCGGGGCCCc -5'
21770 3' -60.7 NC_004813.1 + 46779 0.68 0.365348
Target:  5'- gUCAUCGuaaACUGUGAcgguuaccguCCCGCUggcauaacuguuauuCCGGGGa -3'
miRNA:   3'- -AGUAGCc--UGACACU----------GGGCGG---------------GGCCCC- -5'
21770 3' -60.7 NC_004813.1 + 34468 0.74 0.153431
Target:  5'- ---cUGGugUGUGGCauacgUGCCCCGGGGa -3'
miRNA:   3'- aguaGCCugACACUGg----GCGGGGCCCC- -5'
21770 3' -60.7 NC_004813.1 + 27278 1.11 0.00029
Target:  5'- aUCAUCGGACUGUGACCCGCCCCGGGGg -3'
miRNA:   3'- -AGUAGCCUGACACUGGGCGGGGCCCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.