Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21771 | 3' | -52.8 | NC_004813.1 | + | 2172 | 0.66 | 0.841357 |
Target: 5'- gAGAUACAgGUCUucGCCAUCACCguCa -3' miRNA: 3'- -UCUGUGUaUAGGccUGGUAGUGGguG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 33770 | 0.66 | 0.841357 |
Target: 5'- cGGACGCAcaucAUCCGGggugagacuuuuACCGUCcGCCgGCu -3' miRNA: 3'- -UCUGUGUa---UAGGCC------------UGGUAG-UGGgUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 33932 | 0.66 | 0.841357 |
Target: 5'- cGGACGCAcaucAUCCGGggugagacuuuuACCGUCcGCCgGCu -3' miRNA: 3'- -UCUGUGUa---UAGGCC------------UGGUAG-UGGgUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 34093 | 0.67 | 0.813694 |
Target: 5'- cGGACGCAcaucAUCCGGggugagacuuuuGCCGUCcGCCgGCu -3' miRNA: 3'- -UCUGUGUa---UAGGCC------------UGGUAG-UGGgUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 45003 | 0.67 | 0.804065 |
Target: 5'- ---aGCA-GUCCGGucuUUAUCACCCACa -3' miRNA: 3'- ucugUGUaUAGGCCu--GGUAGUGGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 20986 | 0.67 | 0.794249 |
Target: 5'- cAGGCu----UCCGGACUAcaGCCCACu -3' miRNA: 3'- -UCUGuguauAGGCCUGGUagUGGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 21347 | 0.67 | 0.794249 |
Target: 5'- gAGACAUAUgaccGUCCGGACCcgCguauuaagcaGCUgGCg -3' miRNA: 3'- -UCUGUGUA----UAGGCCUGGuaG----------UGGgUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 30002 | 0.67 | 0.794249 |
Target: 5'- uGACGCAaucCCGGu--GUCACCCGCu -3' miRNA: 3'- uCUGUGUauaGGCCuggUAGUGGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 50232 | 0.68 | 0.742773 |
Target: 5'- --cCACAacugGUCUGGACguUCugCCACg -3' miRNA: 3'- ucuGUGUa---UAGGCCUGguAGugGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 27270 | 0.68 | 0.710401 |
Target: 5'- cAGACG-AUAUCauCGGACUGUgACCCGCc -3' miRNA: 3'- -UCUGUgUAUAG--GCCUGGUAgUGGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 53245 | 0.69 | 0.688402 |
Target: 5'- uGGAC-CGUG-CCGGACgGUCgacggGCCCAUa -3' miRNA: 3'- -UCUGuGUAUaGGCCUGgUAG-----UGGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 34255 | 0.69 | 0.655011 |
Target: 5'- cGGACGCAcaucAUCCGGggugauacuuuuACCGUCugCCGg -3' miRNA: 3'- -UCUGUGUa---UAGGCC------------UGGUAGugGGUg -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 40434 | 0.7 | 0.643823 |
Target: 5'- uGAgGCGUAaUCGGACgCAUUAUCCACc -3' miRNA: 3'- uCUgUGUAUaGGCCUG-GUAGUGGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 47986 | 0.7 | 0.643823 |
Target: 5'- cGuCACAUGcCgGGACCAUCACCg-- -3' miRNA: 3'- uCuGUGUAUaGgCCUGGUAGUGGgug -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 31630 | 0.72 | 0.521096 |
Target: 5'- cAGAUAaAUAUCCGGggcauucaccagcACCAgcuggUCACCCACg -3' miRNA: 3'- -UCUGUgUAUAGGCC-------------UGGU-----AGUGGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 31269 | 0.73 | 0.429634 |
Target: 5'- uGGCAgAUGUcuccCCGGcguCCAUCACCCAUg -3' miRNA: 3'- uCUGUgUAUA----GGCCu--GGUAGUGGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 37374 | 0.73 | 0.429634 |
Target: 5'- cGGGCAUGUA-CCGGACCAUagacggccagCACCUGCu -3' miRNA: 3'- -UCUGUGUAUaGGCCUGGUA----------GUGGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 28727 | 0.75 | 0.364723 |
Target: 5'- cGGACAcCGUAUUCGGAUCggCugCCACc -3' miRNA: 3'- -UCUGU-GUAUAGGCCUGGuaGugGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 24435 | 0.84 | 0.099852 |
Target: 5'- uGGuACGgGUAUCUGGACCAUCugCCGCg -3' miRNA: 3'- -UC-UGUgUAUAGGCCUGGUAGugGGUG- -5' |
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21771 | 3' | -52.8 | NC_004813.1 | + | 28589 | 1.11 | 0.001393 |
Target: 5'- cAGACACAUAUCCGGACCAUCACCCACa -3' miRNA: 3'- -UCUGUGUAUAGGCCUGGUAGUGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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