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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21771 | 5' | -55.3 | NC_004813.1 | + | 28640 | 1.08 | 0.001236 |
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Target: 5'- gUGGUGGCAGCCGAUCCGAAUACGGUGu -3' miRNA: 3'- -ACCACCGUCGGCUAGGCUUAUGCCAC- -5' |
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| 21771 | 5' | -55.3 | NC_004813.1 | + | 41608 | 0.74 | 0.285492 |
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Target: 5'- cGGUGGUccgccGCCGGguguaugcgcguuuUCUGGAUGCGGUGa -3' miRNA: 3'- aCCACCGu----CGGCU--------------AGGCUUAUGCCAC- -5' |
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| 21771 | 5' | -55.3 | NC_004813.1 | + | 21130 | 0.68 | 0.618891 |
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Target: 5'- gGGcUGGCAGCCG-UuuGGuaGUcCGGUGg -3' miRNA: 3'- aCC-ACCGUCGGCuAggCU--UAuGCCAC- -5' |
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| 21771 | 5' | -55.3 | NC_004813.1 | + | 26207 | 0.67 | 0.64086 |
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Target: 5'- cGGUGGCuucacGCaCGGUaCCGGAgcaGGUGg -3' miRNA: 3'- aCCACCGu----CG-GCUA-GGCUUaugCCAC- -5' |
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| 21771 | 5' | -55.3 | NC_004813.1 | + | 30893 | 0.67 | 0.651837 |
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Target: 5'- gUGGgcaGGCAGCCuGUCUGAAUAUcaGGa- -3' miRNA: 3'- -ACCa--CCGUCGGcUAGGCUUAUG--CCac -5' |
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| 21771 | 5' | -55.3 | NC_004813.1 | + | 28512 | 0.67 | 0.688963 |
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Target: 5'- aGGUGGCGGCUaacgccuuuaauccuGcgCgGGAUAUGGUc -3' miRNA: 3'- aCCACCGUCGG---------------CuaGgCUUAUGCCAc -5' |
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| 21771 | 5' | -55.3 | NC_004813.1 | + | 34591 | 0.66 | 0.69438 |
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Target: 5'- cGGUGuGUucagAGCCGGUgaaaccuaccaccCCGGcgAUACGGUGa -3' miRNA: 3'- aCCAC-CG----UCGGCUA-------------GGCU--UAUGCCAC- -5' |
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| 21771 | 5' | -55.3 | NC_004813.1 | + | 45283 | 0.66 | 0.699782 |
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Target: 5'- ---aGGCAGggugcugaccuguauCCGGUCCGGcgcgggguGUGCGGUGa -3' miRNA: 3'- accaCCGUC---------------GGCUAGGCU--------UAUGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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