Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21772 | 3' | -56.4 | NC_004813.1 | + | 17212 | 0.66 | 0.643583 |
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Target: 5'- ---aGCGGUCaacaaggcGAGCgGugaauuCCGGCGACa -3' miRNA: 3'- uuuaCGUCAGa-------CUCGgCu-----GGCCGCUG- -5' |
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| 21772 | 3' | -56.4 | NC_004813.1 | + | 45551 | 0.66 | 0.599366 |
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Target: 5'- ---aGCGGUCagGAcaGCCGuCUGGUGACc -3' miRNA: 3'- uuuaCGUCAGa-CU--CGGCuGGCCGCUG- -5' |
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| 21772 | 3' | -56.4 | NC_004813.1 | + | 48662 | 0.66 | 0.599366 |
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Target: 5'- ---gGCAgGUCcgcagGGGCCGAaaGGUGACa -3' miRNA: 3'- uuuaCGU-CAGa----CUCGGCUggCCGCUG- -5' |
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| 21772 | 3' | -56.4 | NC_004813.1 | + | 41686 | 0.67 | 0.577389 |
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Target: 5'- ---aGCAGgagCUGA-CUGACCGGUGGg -3' miRNA: 3'- uuuaCGUCa--GACUcGGCUGGCCGCUg -5' |
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| 21772 | 3' | -56.4 | NC_004813.1 | + | 32095 | 0.68 | 0.512802 |
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Target: 5'- ---gGCGGUggCUGAGCCcuuuGCCGGCGcCu -3' miRNA: 3'- uuuaCGUCA--GACUCGGc---UGGCCGCuG- -5' |
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| 21772 | 3' | -56.4 | NC_004813.1 | + | 28906 | 0.68 | 0.491913 |
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Target: 5'- --cUGCAguuucuGUCUGAacaccCCGGCUGGCGGCu -3' miRNA: 3'- uuuACGU------CAGACUc----GGCUGGCCGCUG- -5' |
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| 21772 | 3' | -56.4 | NC_004813.1 | + | 42231 | 0.68 | 0.481616 |
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Target: 5'- --cUGCccuGG-CUGAgcgagGCUGACCGGCGGCu -3' miRNA: 3'- uuuACG---UCaGACU-----CGGCUGGCCGCUG- -5' |
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| 21772 | 3' | -56.4 | NC_004813.1 | + | 27738 | 0.7 | 0.412744 |
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Target: 5'- ---gGCuGUCUucGCCGAauaaCCGGCGGCa -3' miRNA: 3'- uuuaCGuCAGAcuCGGCU----GGCCGCUG- -5' |
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| 21772 | 3' | -56.4 | NC_004813.1 | + | 31622 | 1.08 | 0.000802 |
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Target: 5'- gAAAUGCAGUCUGAGCCGACCGGCGACa -3' miRNA: 3'- -UUUACGUCAGACUCGGCUGGCCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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