Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21774 | 5' | -59.5 | NC_004813.1 | + | 56747 | 0.66 | 0.473688 |
Target: 5'- aGGCAaAGAgGCucuUGGCUCagaGGAGCCAa -3' miRNA: 3'- aCCGUgUCUaCG---ACCGGGg--CUUCGGU- -5' |
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21774 | 5' | -59.5 | NC_004813.1 | + | 12903 | 0.67 | 0.398594 |
Target: 5'- cUGGCgGCGGAUuucauucaguucguuCUGGUCCCGGuuAGCCAg -3' miRNA: 3'- -ACCG-UGUCUAc--------------GACCGGGGCU--UCGGU- -5' |
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21774 | 5' | -59.5 | NC_004813.1 | + | 17485 | 0.69 | 0.322144 |
Target: 5'- uUGGCACAu-UGCgccgGGCaCCUGAAGCa- -3' miRNA: 3'- -ACCGUGUcuACGa---CCG-GGGCUUCGgu -5' |
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21774 | 5' | -59.5 | NC_004813.1 | + | 43105 | 1.08 | 0.000369 |
Target: 5'- gUGGCACAGAUGCUGGCCCCGAAGCCAa -3' miRNA: 3'- -ACCGUGUCUACGACCGGGGCUUCGGU- -5' |
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21774 | 5' | -59.5 | NC_004813.1 | + | 6192 | 0.73 | 0.174371 |
Target: 5'- -cGCACGGGuaauggcuuuuuUGCUGGCCCCGuGGCg- -3' miRNA: 3'- acCGUGUCU------------ACGACCGGGGCuUCGgu -5' |
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21774 | 5' | -59.5 | NC_004813.1 | + | 14705 | 0.66 | 0.46378 |
Target: 5'- aUGGCAUGGucaacgGCUGGCgCCgGAAGaCUg -3' miRNA: 3'- -ACCGUGUCua----CGACCG-GGgCUUC-GGu -5' |
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21774 | 5' | -59.5 | NC_004813.1 | + | 31906 | 0.66 | 0.483701 |
Target: 5'- gUGGCACAGccgugugcggagGUGCUGGuuCCCUGGgaaaGGCgGa -3' miRNA: 3'- -ACCGUGUC------------UACGACC--GGGGCU----UCGgU- -5' |
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21774 | 5' | -59.5 | NC_004813.1 | + | 47878 | 0.66 | 0.483701 |
Target: 5'- gUGGUGguGAacacGCUGGCCUCagaaauccggauGAAGCCGg -3' miRNA: 3'- -ACCGUguCUa---CGACCGGGG------------CUUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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